Title |
Scale-up of human embryonic stem cell culture using a hollow fibre bioreactor
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Published in |
Biotechnology Techniques, September 2012
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DOI | 10.1007/s10529-012-1033-1 |
Pubmed ID | |
Authors |
Iwan Roberts, Stefano Baila, R. Brent Rice, Michiel Etienne Janssens, Kim Nguyen, Nathalie Moens, Ludmila Ruban, Diana Hernandez, Pete Coffey, Chris Mason |
Abstract |
The commercialisation of human embryonic stem cell derived cell therapies for large patient populations is reliant on both minimising expensive and variable manual-handling methods whilst realising economies of scale. The Quantum Cell Expansion System, a hollow fibre bioreactor (Terumo BCT), was used in a pilot study to expand 60 million human embryonic stem cells to 708 million cells. Further improvements can be expected with optimisation of media flow rates throughout the run to better control the cellular microenvironment. High levels of pluripotency marker expression were maintained on the bioreactor, with 97.7 % of cells expressing SSEA-4 when harvested. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | <1% |
Portugal | 1 | <1% |
Brazil | 1 | <1% |
Unknown | 99 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 26 | 25% |
Researcher | 16 | 16% |
Student > Bachelor | 12 | 12% |
Student > Master | 11 | 11% |
Student > Doctoral Student | 5 | 5% |
Other | 11 | 11% |
Unknown | 21 | 21% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 17 | 17% |
Agricultural and Biological Sciences | 17 | 17% |
Chemical Engineering | 14 | 14% |
Engineering | 12 | 12% |
Medicine and Dentistry | 8 | 8% |
Other | 7 | 7% |
Unknown | 27 | 26% |