| Title |
Simple isocratic method for simultaneous determination of caffeine and catechins in tea products by HPLC
|
|---|---|
| Published in |
SpringerPlus, July 2016
|
| DOI | 10.1186/s40064-016-2672-9 |
| Pubmed ID | |
| Authors |
Chamira Dilanka Fernando, Preethi Soysa |
| Abstract |
Tea is a popular beverage almost all over the world. Many studies show that tea consumption is closely associated with positive health impact. Most of the HPLC methods used for the determination of tea constituents include gradient elution systems which involve expensive instrumentation. The objective of this study was to develop a simple, rapid precise and low cost HPLC method for the separation and quantification of catechins and caffeine in tea (Camellia sinensis). The method utilizes a phenyl column (2.1 × 150 mm) with a UV-detector (280 nm) where excellent chromatographic separation of tea components i.e. gallic acid (GA), caffeine (Caf), epicatechin (EC) and (-)-epigallocatechin gallate (EGCG) was achieved. The isocratic elution system of acetonitrile, glacial acetic acid and deionized water (8:1:91 v/v/v) at a flow rate of 0.5 mL/min was involved. This method produced excellent accuracy and precision. Within run and between run precision was less than 7.5 %. The equations for calibration curves were y = 0.117 (±0.010)x + 0.173 (±0.024), y = 0.100 (±0.003)x + 0.045 (±0.019), y = 0.016 (±0.001)x + 0.006 (±0.004), y = 0.025 (±0.001)x-0.025 (±0.007) for GA, Caf, EC and EGCG respectively. The method validation parameters prove that the method is efficient, a simple and adequate for the quantitative determination of principal components in tea samples. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 25% |
| Germany | 1 | 25% |
| Mexico | 1 | 25% |
| United States | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 68 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 68 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 22 | 32% |
| Student > Ph. D. Student | 10 | 15% |
| Student > Master | 4 | 6% |
| Researcher | 3 | 4% |
| Student > Doctoral Student | 3 | 4% |
| Other | 10 | 15% |
| Unknown | 16 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 12 | 18% |
| Pharmacology, Toxicology and Pharmaceutical Science | 8 | 12% |
| Agricultural and Biological Sciences | 6 | 9% |
| Biochemistry, Genetics and Molecular Biology | 4 | 6% |
| Nursing and Health Professions | 2 | 3% |
| Other | 12 | 18% |
| Unknown | 24 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 August 2016.
All research outputs
#14,856,861
of 22,880,230 outputs
Outputs from SpringerPlus
#838
of 1,851 outputs
Outputs of similar age
#215,094
of 354,139 outputs
Outputs of similar age from SpringerPlus
#109
of 225 outputs
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