| Title |
A novel Kluyveromyces marxianus strain with an inducible flocculation phenotype
|
|---|---|
| Published in |
AMB Express, July 2012
|
| DOI | 10.1186/2191-0855-2-38 |
| Pubmed ID | |
| Authors |
Juan A Vallejo, Manuel Serrat, Irasema Pérez-Portuondo, Angeles Sánchez-Pérez, Jose M Ageitos, Tomas G Villa |
| Abstract |
Flocculation is a very useful phenotype for industrial yeast strains, since it facilitates cell harvest and represents an easy way of cell immobilization in continuous fermentation processes. The present work represents the first time that an inducible flocculation phenotype has been generated in a non flocculent strain of Kluyveromyces marxianus. This was accomplished by expressing Saccharomyces cerevisiae FLO5 gene in K. marxianus CECT 11769 strain. The FLO 5 gene was placed under the control of an EPG promoter, not repressed by glucose and induced by anoxia. Our experimental approach successfully generated two novel K. marxianus flocculent phenotypes: one inducible and one constitutive. The constitutive phenotype originated from deletions in the FLO5 promoter region, indicating the existence of putative upstream repressor site involved in oxygen regulation of the EPG1 promoter. The novel strains here generated had a unique set of characteristics that provided an advantage, over the wild-type strain, for the industrial co-production of ethanol and polygalacturonase. |
Mendeley readers
The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 2% |
| Unknown | 42 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 16% |
| Researcher | 7 | 16% |
| Student > Ph. D. Student | 7 | 16% |
| Student > Bachelor | 5 | 12% |
| Other | 4 | 9% |
| Other | 5 | 12% |
| Unknown | 8 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 42% |
| Biochemistry, Genetics and Molecular Biology | 8 | 19% |
| Engineering | 4 | 9% |
| Nursing and Health Professions | 1 | 2% |
| Chemical Engineering | 1 | 2% |
| Other | 4 | 9% |
| Unknown | 7 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 July 2012.
All research outputs
#20,163,398
of 22,673,450 outputs
Outputs from AMB Express
#963
of 1,227 outputs
Outputs of similar age
#147,619
of 164,749 outputs
Outputs of similar age from AMB Express
#6
of 6 outputs
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