| Title |
Rhodococcus opacus B4: a promising bacterium for production of biofuels and biobased chemicals
|
|---|---|
| Published in |
AMB Express, May 2016
|
| DOI | 10.1186/s13568-016-0207-y |
| Pubmed ID | |
| Authors |
Ana Rita Castro, Isabel Rocha, Maria Madalena Alves, Maria Alcina Pereira |
| Abstract |
Bacterial lipids have relevant applications in the production of renewable fuels and biobased oleochemicals. The genus Rhodococcus is one of the most relevant lipid producers due to its capability to accumulate those compounds, mainly triacylglycerols (TAG), when cultivated on different defined substrates, namely sugars, organic acids and hydrocarbons but also on complex carbon sources present in industrial wastes. In this work, the production of storage lipids by Rhodococcus opacus B4 using glucose, acetate and hexadecane is reported for the first time and its productivity compared with Rhodococcus opacus PD630, the best TAG producer bacterium reported. Both strains accumulated mainly TAG from all carbon sources, being influenced by the carbon source itself and by the duration of the accumulation period. R. opacus B4 produced 0.09 and 0.14 g L(-1) at 24 and 72 h, with hexadecane as carbon source, which was 2 and 3.3 fold higher than the volumetric production obtained by R. opacus PD630. Both strains presented similar fatty acids (FA) profiles in intact cells while in TAG produced fraction, R. opacus B4 revealed a higher variability in fatty acid composition than R. opacus PD630, when both strains were cultivated on hexadecane. The obtained results open new perspectives for the use of R. opacus B4 to produce TAG, in particular using oily (alkane-contaminated) waste and wastewater as cheap raw-materials. Combining TAG production with hydrocarbons degradation is a promising strategy to achieve environmental remediation while producing added value compounds. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 33% |
| Portugal | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 94 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 1% |
| Unknown | 93 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 18 | 19% |
| Student > Ph. D. Student | 15 | 16% |
| Student > Master | 15 | 16% |
| Student > Bachelor | 12 | 13% |
| Other | 4 | 4% |
| Other | 10 | 11% |
| Unknown | 20 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 20 | 21% |
| Biochemistry, Genetics and Molecular Biology | 16 | 17% |
| Engineering | 10 | 11% |
| Environmental Science | 8 | 9% |
| Chemical Engineering | 4 | 4% |
| Other | 13 | 14% |
| Unknown | 23 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 July 2016.
All research outputs
#15,373,286
of 22,870,727 outputs
Outputs from AMB Express
#445
of 1,235 outputs
Outputs of similar age
#191,078
of 313,736 outputs
Outputs of similar age from AMB Express
#9
of 15 outputs
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