Title |
Standardized Generation and Differentiation of Neural Precursor Cells from Human Pluripotent Stem Cells
|
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Published in |
Stem Cell Reviews and Reports, March 2012
|
DOI | 10.1007/s12015-012-9357-8 |
Pubmed ID | |
Authors |
O. A. Kozhich, R. S. Hamilton, B. S. Mallon |
Abstract |
Precise, robust and scalable directed differentiation of pluripotent stem cells is an important goal with respect to disease modeling or future therapies. Using the AggreWell™400 system we have standardized the differentiation of human embryonic and induced pluripotent stem cells to a neuronal fate using defined conditions. This allows reproducibility in replicate experiments and facilitates the direct comparison of cell lines. Since the starting point for EB formation is a single cell suspension, this protocol is suitable for standard and novel methods of pluripotent stem cell culture. Moreover, an intermediate population of neural precursor cells, which are routinely >95% NCAM(pos) and Tra-1-60(neg) by FACS analysis, may be expanded and frozen prior to differentiation allowing a convenient starting point for downstream experiments. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 1 | 2% |
Unknown | 60 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 13 | 21% |
Student > Ph. D. Student | 12 | 20% |
Student > Bachelor | 10 | 16% |
Student > Master | 9 | 15% |
Student > Doctoral Student | 4 | 7% |
Other | 8 | 13% |
Unknown | 5 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 29 | 48% |
Biochemistry, Genetics and Molecular Biology | 7 | 11% |
Neuroscience | 5 | 8% |
Engineering | 4 | 7% |
Medicine and Dentistry | 3 | 5% |
Other | 6 | 10% |
Unknown | 7 | 11% |