| Title |
Efficient production and secretion of pyruvate from Halomonas sp. KM-1 under aerobic conditions
|
|---|---|
| Published in |
AMB Express, March 2016
|
| DOI | 10.1186/s13568-016-0195-y |
| Pubmed ID | |
| Authors |
Yoshikazu Kawata, Taku Nishimura, Isao Matsushita, Jun Tsubota |
| Abstract |
The alkaliphilic, halophilic bacterium Halomonas sp. KM-1 can utilize both hexose and pentose sugars for the intracellular storage of bioplastic poly-(R)-3-hydroxybutyric acid (PHB) under aerobic conditions. In this study, we investigated the effects of the sodium nitrate concentration on PHB accumulation in the KM-1 strain. Unexpectedly, we observed the secretion of pyruvate, a central intermediate in carbon- and energy-metabolism processes in all organisms; therefore, pyruvate is widely used as a starting material in the industrial biosynthesis of pharmaceuticals and is employed for the production of crop-protection agents, polymers, cosmetics, and food additives. We then further analyzed pyruvate productivity following changes in culture temperature and the buffer concentration. In 48-h batch-cultivation experiments, we found that wild-type Halomonas sp. KM-1 secreted 63.3 g/L pyruvate at a rate of 1.32 g/(L·h), comparable to the results of former studies using mutant and recombinant microorganisms. Thus, these data provided important insights into the production of pyruvate using this novel strain. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 33 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 21% |
| Student > Ph. D. Student | 7 | 21% |
| Student > Bachelor | 4 | 12% |
| Student > Master | 3 | 9% |
| Other | 3 | 9% |
| Other | 3 | 9% |
| Unknown | 6 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 30% |
| Biochemistry, Genetics and Molecular Biology | 7 | 21% |
| Chemical Engineering | 2 | 6% |
| Chemistry | 2 | 6% |
| Immunology and Microbiology | 1 | 3% |
| Other | 4 | 12% |
| Unknown | 7 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 March 2016.
All research outputs
#3,240,769
of 22,856,968 outputs
Outputs from AMB Express
#49
of 1,235 outputs
Outputs of similar age
#53,887
of 300,781 outputs
Outputs of similar age from AMB Express
#3
of 25 outputs
Altmetric has tracked 22,856,968 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,235 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done particularly well, scoring higher than 95% of its peers.
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We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.