Title |
Automation of antimicrobial activity screening
|
---|---|
Published in |
AMB Express, March 2016
|
DOI | 10.1186/s13568-016-0191-2 |
Pubmed ID | |
Authors |
Samuel P. Forry, Megan C. Madonna, Daneli López-Pérez, Nancy J. Lin, Madeleine D. Pasco |
Abstract |
Manual and automated methods were compared for routine screening of compounds for antimicrobial activity. Automation generally accelerated assays and required less user intervention while producing comparable results. Automated protocols were validated for planktonic, biofilm, and agar cultures of the oral microbe Streptococcus mutans that is commonly associated with tooth decay. Toxicity assays for the known antimicrobial compound cetylpyridinium chloride (CPC) were validated against planktonic, biofilm forming, and 24 h biofilm culture conditions, and several commonly reported toxicity/antimicrobial activity measures were evaluated: the 50 % inhibitory concentration (IC50), the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC). Using automated methods, three halide salts of cetylpyridinium (CPC, CPB, CPI) were rapidly screened with no detectable effect of the counter ion on antimicrobial activity. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Brazil | 1 | 17% |
United States | 1 | 17% |
Switzerland | 1 | 17% |
Unknown | 3 | 50% |
Demographic breakdown
Type | Count | As % |
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Scientists | 3 | 50% |
Members of the public | 3 | 50% |
Mendeley readers
Geographical breakdown
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France | 1 | 3% |
Unknown | 33 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 5 | 15% |
Student > Master | 4 | 12% |
Student > Postgraduate | 4 | 12% |
Researcher | 4 | 12% |
Student > Ph. D. Student | 4 | 12% |
Other | 9 | 26% |
Unknown | 4 | 12% |
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Agricultural and Biological Sciences | 7 | 21% |
Chemistry | 6 | 18% |
Medicine and Dentistry | 4 | 12% |
Immunology and Microbiology | 2 | 6% |
Other | 5 | 15% |
Unknown | 3 | 9% |