| Title |
Automation of antimicrobial activity screening
|
|---|---|
| Published in |
AMB Express, March 2016
|
| DOI | 10.1186/s13568-016-0191-2 |
| Pubmed ID | |
| Authors |
Samuel P. Forry, Megan C. Madonna, Daneli López-Pérez, Nancy J. Lin, Madeleine D. Pasco |
| Abstract |
Manual and automated methods were compared for routine screening of compounds for antimicrobial activity. Automation generally accelerated assays and required less user intervention while producing comparable results. Automated protocols were validated for planktonic, biofilm, and agar cultures of the oral microbe Streptococcus mutans that is commonly associated with tooth decay. Toxicity assays for the known antimicrobial compound cetylpyridinium chloride (CPC) were validated against planktonic, biofilm forming, and 24 h biofilm culture conditions, and several commonly reported toxicity/antimicrobial activity measures were evaluated: the 50 % inhibitory concentration (IC50), the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC). Using automated methods, three halide salts of cetylpyridinium (CPC, CPB, CPI) were rapidly screened with no detectable effect of the counter ion on antimicrobial activity. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 17% |
| United States | 1 | 17% |
| Switzerland | 1 | 17% |
| Unknown | 3 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 3 | 50% |
| Members of the public | 3 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 3% |
| Unknown | 33 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 5 | 15% |
| Student > Master | 4 | 12% |
| Student > Postgraduate | 4 | 12% |
| Researcher | 4 | 12% |
| Student > Ph. D. Student | 4 | 12% |
| Other | 9 | 26% |
| Unknown | 4 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 21% |
| Agricultural and Biological Sciences | 7 | 21% |
| Chemistry | 6 | 18% |
| Medicine and Dentistry | 4 | 12% |
| Immunology and Microbiology | 2 | 6% |
| Other | 5 | 15% |
| Unknown | 3 | 9% |
Attention Score in Context
This research output has an Altmetric Attention Score of 83. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 July 2016.
All research outputs
#431,498
of 22,856,968 outputs
Outputs from AMB Express
#4
of 1,235 outputs
Outputs of similar age
#8,748
of 300,258 outputs
Outputs of similar age from AMB Express
#1
of 24 outputs
Altmetric has tracked 22,856,968 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,235 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 300,258 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.