Title |
Microbial degradation of the morphine alkaloids: identification of morphinone as an intermediate in the metabolism of morphine by Pseudomonas putida M10
|
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Published in |
Archives of Microbiology, October 1990
|
DOI | 10.1007/bf00245229 |
Pubmed ID | |
Authors |
Neil C. Bruce, Clare J. Wilmot, Keith N. Jordan, Anna E. Trebilcock, Lauren D. Gray Stephens, Christopher R. Lowe |
Abstract |
A strain of Pseudomonas putida was isolated by selective enrichment with morphine that was capable of utilising morphine as a primary source of carbon and energy for growth. Experiments with whole cells showed that both morphine and codeine, but not thebaine, could be utilised. A novel NADP-dependent dehydrogenase, morphine dehydrogenase, was purified from crude cell extracts and was shown to be capable of oxidising morphine and codeine to morphinone and codeinone, respectively. This NADP-dependent morphine dehydrogenase was not observed in any other species of pseudomonads examined and was quite distinct from the beta-hydroxysteroid dehydrogenase found in Pseudomonas testosteroni, which had previously been shown to have activity against morphine. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Austria | 1 | 4% |
Unknown | 23 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 29% |
Student > Ph. D. Student | 5 | 21% |
Unspecified | 3 | 13% |
Student > Master | 3 | 13% |
Other | 1 | 4% |
Other | 2 | 8% |
Unknown | 3 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 10 | 42% |
Biochemistry, Genetics and Molecular Biology | 4 | 17% |
Unspecified | 3 | 13% |
Environmental Science | 2 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Other | 0 | 0% |
Unknown | 4 | 17% |