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Python Erythrocytes Are Resistant to α-Hemolysin from Escherichia coli

Overview of attention for article published in Journal of Membrane Biology, November 2011
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  • Good Attention Score compared to outputs of the same age (68th percentile)

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28 Mendeley
Title
Python Erythrocytes Are Resistant to α-Hemolysin from Escherichia coli
Published in
Journal of Membrane Biology, November 2011
DOI 10.1007/s00232-011-9406-2
Pubmed ID
Authors

Casper K. Larsen, Marianne Skals, Tobias Wang, Muhammad U. Cheema, Jens Leipziger, Helle A. Praetorius

Abstract

α-Hemolysin (HlyA) from Escherichia coli lyses mammalian erythrocytes by creating nonselective cation pores in the membrane. Pore insertion triggers ATP release and subsequent P2X receptor and pannexin channel activation. Blockage of either P2X receptors or pannexin channels reduces HlyA-induced hemolysis. We found that erythrocytes from Python regius and Python molurus are remarkably resistant to HlyA-induced hemolysis compared to human and Trachemys scripta erythrocytes. HlyA concentrations that induced maximal hemolysis of human erythrocytes did not affect python erythrocytes, but increasing the HlyA concentration 40-fold did induce hemolysis. Python erythrocytes were more resistant to osmotic stress than human erythrocytes, but osmotic stress tolerance per se did not confer HlyA resistance. Erythrocytes from T. scripta, which showed higher osmotic resistance than python erythrocytes, were as susceptible to HlyA as human erythrocytes. Therefore, we tested whether python erythrocytes lack the purinergic signalling known to amplify HlyA-induced hemolysis in human erythrocytes. P. regius erythrocytes increased intracellular Ca²⁺ concentration and reduced cell volume when exposed to 3 mM ATP, indicating the presence of a P2X₇-like receptor. In addition, scavenging extracellular ATP or blocking P2 receptors or pannexin channels reduced the HlyA-induced hemolysis. We tested whether the low HlyA sensitivity resulted from low affinity of HlyA to the python erythrocyte membrane. We found comparable incorporation of HlyA into human and python erythrocyte membranes. Taken together, the remarkable HlyA resistance of python erythrocytes was not explained by increased osmotic resistance, lack of purinergic hemolysis amplification, or differences in HlyA affinity.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 5 18%
France 1 4%
Germany 1 4%
Unknown 21 75%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 29%
Professor 3 11%
Student > Bachelor 3 11%
Student > Master 3 11%
Student > Doctoral Student 3 11%
Other 8 29%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 21%
Medicine and Dentistry 4 14%
Physics and Astronomy 3 11%
Computer Science 3 11%
Philosophy 2 7%
Other 10 36%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 December 2011.
All research outputs
#1,879,178
of 4,505,992 outputs
Outputs from Journal of Membrane Biology
#103
of 201 outputs
Outputs of similar age
#72,317
of 235,163 outputs
Outputs of similar age from Journal of Membrane Biology
#1
of 2 outputs
Altmetric has tracked 4,505,992 research outputs across all sources so far. This one has received more attention than most of these and is in the 57th percentile.
So far Altmetric has tracked 201 research outputs from this source. They receive a mean Attention Score of 2.0. This one is in the 48th percentile – i.e., 48% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 235,163 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them