| Title |
Disruption of PHO13 improves ethanol production via the xylose isomerase pathway
|
|---|---|
| Published in |
AMB Express, January 2016
|
| DOI | 10.1186/s13568-015-0175-7 |
| Pubmed ID | |
| Authors |
Takahiro Bamba, Tomohisa Hasunuma, Akihiko Kondo |
| Abstract |
Xylose is the second most abundant sugar in lignocellulosic materials and can be converted to ethanol by recombinant Saccharomyces cerevisiae yeast strains expressing heterologous genes involved in xylose assimilation pathways. Recent research demonstrated that disruption of the alkaline phosphatase gene, PHO13, enhances ethanol production from xylose by a strain expressing the xylose reductase (XR) and xylitol dehydrogenase (XDH) genes; however, the yield of ethanol is poor. In this study, PHO13 was disrupted in a recombinant strain harboring multiple copies of the xylose isomerase (XI) gene derived from Orpinomyces sp., coupled with overexpression of the endogenous xylulokinase (XK) gene and disruption of GRE3, which encodes aldose reductase. The resulting YΔGP/XK/XI strain consumed 2.08 g/L/h of xylose and produced 0.88 g/L/h of volumetric ethanol, for an 86.8 % theoretical ethanol yield, and only YΔGP/XK/XI demonstrated increase in cell concentration. Transcriptome analysis indicated that expression of genes involved in the pentose phosphate pathway (GND1, SOL3, TAL1, RKI1, and TKL1) and TCA cycle and respiratory chain (NDE1, ACO1, ACO2, SDH2, IDH1, IDH2, ATP7, ATP19, SDH4, SDH3, CMC2, and ATP15) was upregulated in the YΔGP/XK/XI strain. And the expression levels of 125 cell cycle genes were changed by deletion of PHO13. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 1% |
| Unknown | 68 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 17 | 25% |
| Student > Ph. D. Student | 15 | 22% |
| Student > Master | 10 | 14% |
| Student > Bachelor | 6 | 9% |
| Student > Doctoral Student | 5 | 7% |
| Other | 5 | 7% |
| Unknown | 11 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 23 | 33% |
| Biochemistry, Genetics and Molecular Biology | 21 | 30% |
| Chemical Engineering | 5 | 7% |
| Chemistry | 3 | 4% |
| Engineering | 2 | 3% |
| Other | 2 | 3% |
| Unknown | 13 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 January 2016.
All research outputs
#12,941,290
of 22,840,638 outputs
Outputs from AMB Express
#227
of 1,234 outputs
Outputs of similar age
#180,112
of 395,720 outputs
Outputs of similar age from AMB Express
#5
of 27 outputs
Altmetric has tracked 22,840,638 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,234 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done well, scoring higher than 81% of its peers.
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We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.