| Title |
Molecular breeding of lignin-degrading brown-rot fungus Gloeophyllum trabeum by homologous expression of laccase gene
|
|---|---|
| Published in |
AMB Express, December 2015
|
| DOI | 10.1186/s13568-015-0173-9 |
| Pubmed ID | |
| Authors |
Misa Arimoto, Kenji Yamagishi, Jianqiao Wang, Kanade Tanaka, Takanori Miyoshi, Ichiro Kamei, Ryuichiro Kondo, Toshio Mori, Hirokazu Kawagishi, Hirofumi Hirai |
| Abstract |
The basidiomycete Gloeophyllum trabeum KU-41 can degrade Japanese cedar wood efficiently. To construct a strain better suited for biofuel production from Japanese cedar wood, we developed a gene transformation system for G. trabeum KU-41 using the hygromycin phosphotransferase-encoding gene (hpt) as a marker. The endogenous laccase candidate gene (Gtlcc3) was fused with the promoter of the G. trabeum glyceraldehyde-3-phosphate dehydrogenase-encoding gene and co-transformed with the hpt-bearing pAH marker plasmid. We obtained 44 co-transformants, and identified co-transformant L#61, which showed the highest laccase activity among all the transformants. Moreover, strain L#61 was able to degrade lignin in Japanese cedar wood-containing medium, in contrast to wild-type G. trabeum KU-41 and to a typical white-rot fungus Phanerochaete chrysosporium. By using strain L#61, direct ethanol production from Japanese cedar wood was improved compared to wild type. To our knowledge, this study is the first report of the molecular breeding of lignin-degrading brown-rot fungus and direct ethanol production from softwoods by co-transformation with laccase overproduction constructs. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 3% |
| Unknown | 30 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 16% |
| Professor | 4 | 13% |
| Researcher | 4 | 13% |
| Student > Master | 3 | 10% |
| Professor > Associate Professor | 2 | 6% |
| Other | 3 | 10% |
| Unknown | 10 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 39% |
| Biochemistry, Genetics and Molecular Biology | 3 | 10% |
| Earth and Planetary Sciences | 1 | 3% |
| Materials Science | 1 | 3% |
| Engineering | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 13 | 42% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 December 2015.
All research outputs
#15,352,477
of 22,836,570 outputs
Outputs from AMB Express
#445
of 1,234 outputs
Outputs of similar age
#228,913
of 390,618 outputs
Outputs of similar age from AMB Express
#13
of 27 outputs
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