| Title |
Identification of the nucleophile catalytic residue of GH51 α-l-arabinofuranosidase from Pleurotus ostreatus
|
|---|---|
| Published in |
AMB Express, December 2015
|
| DOI | 10.1186/s13568-015-0164-x |
| Pubmed ID | |
| Authors |
Antonella Amore, Alfonso Iadonisi, Florence Vincent, Vincenza Faraco |
| Abstract |
In this study, the recombinant α-L-arabinofuranosidase from the fungus Pleurotus ostreatus (rPoAbf) was subjected to site-directed mutagenesis in order to identify the catalytic nucleophile residue. Based on bioinformatics and homology modelling analyses, E449 was revealed to be the potential nucleophilic residue. Thus, the mutant E449G of PoAbf was recombinantly expressed in Pichia pastoris and its recombinant expression level and reactivity were investigated in comparison to the wild-type. The design of a suitable set of hydrolysis experiments in the presence or absence of alcoholic arabinosyl acceptors and/or formate salts allowed to unambiguously identify the residue E449 as the nucleophile residue involved in the retaining mechanism of this GH51 arabinofuranosidase. (1)H NMR analysis was applied for the identification of the products and the assignement of their anomeric configuration. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 2% |
| Unknown | 44 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 13% |
| Student > Doctoral Student | 2 | 4% |
| Researcher | 2 | 4% |
| Student > Bachelor | 1 | 2% |
| Professor | 1 | 2% |
| Other | 3 | 7% |
| Unknown | 30 | 67% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 16% |
| Chemistry | 3 | 7% |
| Biochemistry, Genetics and Molecular Biology | 2 | 4% |
| Environmental Science | 1 | 2% |
| Unknown | 32 | 71% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 December 2015.
All research outputs
#15,352,477
of 22,836,570 outputs
Outputs from AMB Express
#445
of 1,234 outputs
Outputs of similar age
#228,307
of 389,451 outputs
Outputs of similar age from AMB Express
#12
of 26 outputs
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