| Title |
Purification and characterization of an amyloglucosidase from an ericoid mycorrhizal fungus (Leohumicola incrustata)
|
|---|---|
| Published in |
AMB Express, September 2018
|
| DOI | 10.1186/s13568-018-0685-1 |
| Pubmed ID | |
| Authors |
O. R. Adeoyo, B. I. Pletschke, J. F. Dames |
| Abstract |
This study aimed to purify and characterize amyloglucosidase (AMG) from Leohumicola incrustata. AMG was purified to homogeneity from cell-free culture filtrate of an ERM fungus grown in a modified Melin-Norkrans liquid medium. The molecular mass of the AMG was estimated to be 101 kDa by combining the results of Sephadex G-100 gel filtration, sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and zymography. The Km and kcat values were 0.38 mg mL-1 and 70 s-1, respectively, using soluble starch as a substrate. The enzyme was stable at 45 °C (pH 5.0), retaining over 65% activity after a pre-incubation period of 24 h. The metal inhibition profile of the AMG showed that Mn2+ and Ca2+ enhanced activity, while it was stable to metals ions, except a few (Al3+, Co2+, Hg2+ and Cd2+) that were inhibitory at a concentration higher than 5 mM. Thin layer chromatography revealed that only glucose was produced as the product of starch hydrolysis. The amylase from L. incrustata is a glucoamylase with promising characteristics such as temperature stability over an extended period, high substrate affinity and stability to a range of chemicals. Also, this study reports for the first time the possibility of using some culturable ERM fungi to produce enzymes for the bio-economy. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| South Africa | 1 | 33% |
| Germany | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 60 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 11 | 18% |
| Student > Ph. D. Student | 8 | 13% |
| Researcher | 6 | 10% |
| Student > Master | 3 | 5% |
| Unspecified | 2 | 3% |
| Other | 4 | 7% |
| Unknown | 26 | 43% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 23% |
| Biochemistry, Genetics and Molecular Biology | 4 | 7% |
| Unspecified | 2 | 3% |
| Environmental Science | 2 | 3% |
| Nursing and Health Professions | 2 | 3% |
| Other | 9 | 15% |
| Unknown | 27 | 45% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 November 2018.
All research outputs
#15,546,615
of 23,105,443 outputs
Outputs from AMB Express
#448
of 1,245 outputs
Outputs of similar age
#214,727
of 342,007 outputs
Outputs of similar age from AMB Express
#12
of 53 outputs
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We're also able to compare this research output to 53 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.