Title |
Characterisation of a newly detected bacteriophage infecting Bordetella bronchiseptica in swine
|
---|---|
Published in |
Archives of Virology, September 2018
|
DOI | 10.1007/s00705-018-4034-0 |
Pubmed ID | |
Authors |
Yibao Chen, Lan Yang, Erchao Sun, Jiaoyang Song, Bin Wu |
Abstract |
A novel virulent bacteriophage, vB_BbrM_PHB04, infecting Bordetella bronchiseptica was isolated from wastewater collected at a swine farm in China. Phage vB_BbrM_PHB04 exhibited growth over a wide range of temperature and pH conditions and showed different efficiency of plating values and lytic spectra within the same strains at 25 °C and 37 °C. High-throughput sequencing revealed that vB_BbrM_PHB04 has a linear double-stranded DNA genome with 124 putative open reading frames. Overall, the genome of vB_BbrM_PHB04 showed very low similarity (the highest nucleotide identity 82%, 1% coverage) to other phage sequences in the GenBank database. Phylogenetic analysis indicated that vB_BbrM_PHB04 is a new member of the family Myoviridae. In addition, polymerase chain reaction-based detection of phage genes in phage-resistant B. bronchiseptica variants revealed no evidence of lysogenic activity of phage vB_BbrM_PHB04. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 24 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 13% |
Student > Master | 3 | 13% |
Student > Ph. D. Student | 2 | 8% |
Researcher | 2 | 8% |
Librarian | 1 | 4% |
Other | 1 | 4% |
Unknown | 12 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 21% |
Biochemistry, Genetics and Molecular Biology | 2 | 8% |
Veterinary Science and Veterinary Medicine | 1 | 4% |
Environmental Science | 1 | 4% |
Immunology and Microbiology | 1 | 4% |
Other | 1 | 4% |
Unknown | 13 | 54% |