| Title |
The heat shock protein 70 gene as a new alternative molecular marker for the taxonomic identification of Streptomyces strains
|
|---|---|
| Published in |
AMB Express, September 2018
|
| DOI | 10.1186/s13568-018-0674-4 |
| Pubmed ID | |
| Authors |
Yang Hu, Fengxian Sun, Weiying Liu |
| Abstract |
With the developments in taxonomy, the classically used highly conserved 16S rRNA molecular marker has shown some disadvantages among closely related species. For further taxonomic studies of the prokaryotes, specific PCR primers were designed from two conserved regions in the amino acid sequences of the 70-kDa heat shock protein sourced from 20 different genera in actinomycetes. These were used for the amplification of the hsp70 genes in 16 Streptomyces strains. Then, we investigated the phylogenetic relationships among these Streptomyces strains and compared the tree topology based on the hsp70 gene with those based on the previously used markers (16S rRNA and gyrB). To our knowledge, this is the first use of the hsp70 gene as a molecular marker for the taxonomic identification of Streptomyces. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 39% |
| Student > Bachelor | 2 | 11% |
| Unspecified | 1 | 6% |
| Student > Doctoral Student | 1 | 6% |
| Professor | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 5 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 44% |
| Biochemistry, Genetics and Molecular Biology | 4 | 22% |
| Unspecified | 1 | 6% |
| Environmental Science | 1 | 6% |
| Unknown | 4 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 September 2018.
All research outputs
#18,649,291
of 23,103,436 outputs
Outputs from AMB Express
#808
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Outputs of similar age
#258,917
of 337,287 outputs
Outputs of similar age from AMB Express
#18
of 53 outputs
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