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A reevaluation of iron binding by Mycobactin J

Overview of attention for article published in Journal of Biological Inorganic Chemistry, July 2018
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6 Mendeley
Title
A reevaluation of iron binding by Mycobactin J
Published in
Journal of Biological Inorganic Chemistry, July 2018
DOI 10.1007/s00775-018-1592-2
Pubmed ID
Authors

Courtney F. McQueen, John T. Groves

Abstract

The complex stability constant (log β110) and the free iron concentration (pM) are used to compare the relative strength of iron binding by siderophores. Direct measurements of these thermodynamic parameters are often not possible for siderophores due to very large log β110 values ranging from 30 to 50. Instead, siderophore iron(III)-binding constants are determined by competitive experiments with other strong chelators with known values, such as EDTA. Iron(III) binding constants of water-insoluble siderophores, such as the mycobactins produced by the mycobacterium family, have never been directly measured. Since mycobactins contain two hydroxamic acid binding motifs, their log β110 values have been assumed to be comparable to those of other hydroxamate-based siderophores like desferrioxamine B, at ~ 30. However, exochelin MN, another mycobacterial siderophore that contains two hydroxamic acid moieties, has a log β110 of 39.1 and a pM of 31.1, which makes it among the strongest siderophores known. We have found that mycobactin J, the amphiphilic siderophore of Mycobacterium paratuberculosis, can remove iron(III) from TrenCAM (log β110 = 43.6) within 1 min in methanol. This surprising result indicates that log β110 for mycobactin J is ~ 43 and the ligand exchange kinetics in methanol is fast. The results imply that mycobactins are capable of removing iron quickly from very strongly binding siderophores in a cellular milieu. We propose a model mechanism for iron acquisition by pathogenic mycobacteria in vivo. This model explains how the host iron captured by siderophores can be returned to the invading pathogen even in the absence of active uptake mechanisms.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 17%
Student > Master 1 17%
Student > Ph. D. Student 1 17%
Student > Doctoral Student 1 17%
Researcher 1 17%
Other 1 17%
Readers by discipline Count As %
Unspecified 3 50%
Biochemistry, Genetics and Molecular Biology 1 17%
Agricultural and Biological Sciences 1 17%
Chemistry 1 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 July 2018.
All research outputs
#7,466,140
of 12,384,948 outputs
Outputs from Journal of Biological Inorganic Chemistry
#237
of 372 outputs
Outputs of similar age
#147,015
of 268,542 outputs
Outputs of similar age from Journal of Biological Inorganic Chemistry
#2
of 6 outputs
Altmetric has tracked 12,384,948 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 372 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 34th percentile – i.e., 34% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 268,542 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.