| Title |
Production of a modified peptide clavanin in Pichia pastoris: cloning, expression, purification and in vitro activities
|
|---|---|
| Published in |
AMB Express, August 2015
|
| DOI | 10.1186/s13568-015-0129-0 |
| Pubmed ID | |
| Authors |
Kelly Cristina Mulder, Loiane Alves de Lima, Priscilla Santos Aguiar, Fábio Correa Carneiro, Octávio Luiz Franco, Simoni Campos Dias, Nádia Skorupa Parachin |
| Abstract |
Antimicrobial peptides are one of the most promising peptide-based drugs due to their enormous potential as novel biopharmaceuticals in both human and animal industries. In order to develop strategies to over produce such molecules, heterologous production of a modified version of clavanin A, here named clavanin MO (clavMO), was successfully achieved in the methylothopic yeast Pichia pastoris. ClavMO was fused to thioredoxin as a carrier protein and the construction was tested using two promoters, PAOX1 and PGAP, based on either induced or constitutive expression systems, respectively. After growth in 5 L Bioreactor, clavMO-thio was recovered and purified through size exclusion chromatography. Our findings show that both constitutive and inducible expression systems produce active clavMO fused to thioredoxin against both Gram-negative Klebsiella pneumoniae and Gram-positive Staphylococcus aureus microorganisms. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 33% |
| Australia | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 64 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 12 | 19% |
| Researcher | 6 | 9% |
| Student > Ph. D. Student | 6 | 9% |
| Student > Bachelor | 5 | 8% |
| Professor > Associate Professor | 5 | 8% |
| Other | 15 | 23% |
| Unknown | 15 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 20 | 31% |
| Agricultural and Biological Sciences | 16 | 25% |
| Engineering | 3 | 5% |
| Chemistry | 3 | 5% |
| Business, Management and Accounting | 2 | 3% |
| Other | 5 | 8% |
| Unknown | 15 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 August 2015.
All research outputs
#14,234,315
of 22,821,814 outputs
Outputs from AMB Express
#302
of 1,234 outputs
Outputs of similar age
#136,061
of 264,036 outputs
Outputs of similar age from AMB Express
#6
of 25 outputs
Altmetric has tracked 22,821,814 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,234 research outputs from this source. They receive a mean Attention Score of 2.8. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,036 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.