| Title |
Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli
|
|---|---|
| Published in |
AMB Express, June 2018
|
| DOI | 10.1186/s13568-018-0624-1 |
| Pubmed ID | |
| Authors |
Xian Xu, Liqing Tian, Jiali Xu, Chengjia Xie, Ling Jiang, He Huang |
| Abstract |
Deinococcus wulumuqiensis R12 is a red-pigmented extremophilic microorganism with powerful antioxidant properties that was isolated from radiation-contaminated soil in Xinjiang Uyghur Autonomous Region of China. The key carotenoid biosynthesis genes, crtE, crtB and crtI, which are related to the cells' antioxidant defense, were identified in the sequenced genome of R12 and analyzed. In order to improve the carotenoid yield in engineered Escherichia coli, the origin of carotenoid biosynthesis genes was discussed, and a strain containing the R12 carotenoid biosynthesis genes was constructed to produce lycopene, an important intermediate in carotenoid metabolism. The gene order and fermentation conditions, including the culture medium, temperature, and light, were optimized to obtain a genetically engineered strain with a high lycopene production capacity. The highest lycopene content was 688 mg L-1 in strain IEB, which corresponds to a 2.2-fold improvement over the original recombinant strain EBI. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 50 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 22% |
| Student > Bachelor | 8 | 16% |
| Student > Master | 4 | 8% |
| Student > Doctoral Student | 2 | 4% |
| Student > Ph. D. Student | 2 | 4% |
| Other | 4 | 8% |
| Unknown | 19 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 20% |
| Agricultural and Biological Sciences | 7 | 14% |
| Immunology and Microbiology | 2 | 4% |
| Chemical Engineering | 2 | 4% |
| Computer Science | 1 | 2% |
| Other | 9 | 18% |
| Unknown | 19 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 June 2018.
All research outputs
#20,518,141
of 23,085,832 outputs
Outputs from AMB Express
#974
of 1,245 outputs
Outputs of similar age
#289,385
of 329,907 outputs
Outputs of similar age from AMB Express
#30
of 61 outputs
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