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Haptotaxis is Cell Type Specific and Limited by Substrate Adhesiveness

Overview of attention for article published in Cellular and Molecular Bioengineering, June 2015
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26 Mendeley
Title
Haptotaxis is Cell Type Specific and Limited by Substrate Adhesiveness
Published in
Cellular and Molecular Bioengineering, June 2015
DOI 10.1007/s12195-015-0398-3
Pubmed ID
Authors

Jessica H. Wen, Onkiu Choi, Hermes Taylor-Weiner, Alexander Fuhrmann, Jerome V. Karpiak, Adah Almutairi, Adam J. Engler

Abstract

Motile cells navigate through tissue by relying on tactile cues from gradients provided by extracellular matrix (ECM) such as ligand density or stiffness. Mesenchymal stem cells (MSCs) and fibroblasts encounter adhesive or 'haptotactic' gradients at the interface between healthy and fibrotic tissue as they migrate towards an injury site. Mimicking this phenomenon, we developed tunable RGD and collagen gradients in polyacrylamide hydrogels of physiologically relevant stiffness using density gradient multilayer polymerization (DGMP) to better understand how such ligand gradients regulate migratory behaviors. Independent of ligand composition and fiber deformation, haptotaxis was observed in mouse 3T3 fibroblasts. Human MSCs however, haptotaxed only when cell-substrate adhesion was indirectly reduced via addition of free soluble matrix ligand mimetic peptides. Under basal conditions, MSCs were more contractile than fibroblasts. However, the presence of soluble adhesive peptides reduced MSC-induced substrate deformations; increased contractility may contribute to limited migration, but modulating cytoskeletal assembly was ineffective at promoting MSC haptotaxis. When introduced to gradients of increased absolute ligand concentrations, 3T3s displayed increased contractility and no longer haptotaxed. These data suggest that haptotactic behaviors are limited by adhesion and that although both cell types may home to tissue to aid in repair, fibroblasts may be more responsive to ligand gradients than MSCs.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 12 46%
Student > Bachelor 3 12%
Student > Doctoral Student 3 12%
Unspecified 2 8%
Researcher 2 8%
Other 4 15%
Readers by discipline Count As %
Engineering 8 31%
Agricultural and Biological Sciences 7 27%
Biochemistry, Genetics and Molecular Biology 4 15%
Unspecified 2 8%
Materials Science 2 8%
Other 3 12%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 June 2015.
All research outputs
#9,973,658
of 13,040,640 outputs
Outputs from Cellular and Molecular Bioengineering
#181
of 280 outputs
Outputs of similar age
#121,138
of 194,936 outputs
Outputs of similar age from Cellular and Molecular Bioengineering
#9
of 18 outputs
Altmetric has tracked 13,040,640 research outputs across all sources so far. This one is in the 20th percentile – i.e., 20% of other outputs scored the same or lower than it.
So far Altmetric has tracked 280 research outputs from this source. They receive a mean Attention Score of 2.5. This one is in the 12th percentile – i.e., 12% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 194,936 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one is in the 5th percentile – i.e., 5% of its contemporaries scored the same or lower than it.