In the present study, we have designed a press-coated pulsatile delivery tablet (PDT) of Terbutaline sulphate (TS) intended for prevention of early-morning asthma attacks. The formulation is capable of giving burst release of drug after 6 h of lag time. In this study, a press-coating technique was employed to coat a fast-release core tablet containing TS with a polymeric release retarding coat comprising of ethyl cellulose, HPMC K15M and Carbopol 971P. Fast-release core tablets were formulated using sodium starch glycolate with croscarmellose sodium (disintegrants). Optimization of PDT was done by response surface methodology employing the Box-Behnken design. The formulations underwent physical evaluation and drug release study. A lag time of 6 h was obtained by the optimized PDT formulation (OP1). The accelerated stability studies showed no significant changes in physicochemical properties and release behaviour before and after storage. Further in vivo pharmacokinetic studies were performed on rabbits to determine pharmacokinetic parameters. The formulation OP1 showed C max and T max values of 162.92 ± 9.85 ng/mL and 10 h, respectively. A large value of MRT for the formulation OP1 (12.34 ± 0.778 h) signifies that formulation OP1 was able to delay the release. The study concludes that the chronotherapeutic PDT of TS can be successfully formulated and used to provide a pulsatile release for the chronotherapy of nocturnal asthma.