| Title |
Evaluation of anthocyanins in Aronia melanocarpa/BSA binding by spectroscopic studies
|
|---|---|
| Published in |
AMB Express, May 2018
|
| DOI | 10.1186/s13568-018-0604-5 |
| Pubmed ID | |
| Authors |
Jie Wei, Dexin Xu, Xiao Zhang, Jing Yang, Qiuyu Wang |
| Abstract |
The interaction between Anthocyanins in Aronia melanocarpa (AMA) and bovine serum albumin (BSA) were studied in this paper by multispectral technology, such as fluorescence quenching titration, circular dichroism (CD) spectroscopy and Fourier transform infrared spectroscopy (FTIR). The results of the fluorescence titration revealed that AMA could strongly quench the intrinsic fluorescence of BSA by static quenching. The apparent binding constants KSV and number of binding sites n of AMA with BSA were obtained by fluorescence quenching method. The thermodynamic parameters, enthalpy change (ΔH) and entropy change (ΔS), were calculated to be 18.45 kJ mol-1 > 0 and 149.72 J mol-1 K-1 > 0, respectively, which indicated that the interaction of AMA with BSA was driven mainly by hydrophobic forces. The binding process was a spontaneous process of Gibbs free energy change. Based on Förster's non-radiative energy transfer theory, the distance r between the donor (BSA) and the receptor (AMA) was calculated to be 3.88 nm. Their conformations were analyzed using infrared spectroscopy and CD. The results of multispectral technology showed that the binding of AMA to BSA induced the conformational change of BSA. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 20% |
| Student > Ph. D. Student | 4 | 20% |
| Student > Bachelor | 2 | 10% |
| Researcher | 2 | 10% |
| Student > Doctoral Student | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 6 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 4 | 20% |
| Agricultural and Biological Sciences | 2 | 10% |
| Business, Management and Accounting | 1 | 5% |
| Chemical Engineering | 1 | 5% |
| Physics and Astronomy | 1 | 5% |
| Other | 3 | 15% |
| Unknown | 8 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 May 2018.
All research outputs
#20,485,225
of 23,047,237 outputs
Outputs from AMB Express
#974
of 1,242 outputs
Outputs of similar age
#287,292
of 326,328 outputs
Outputs of similar age from AMB Express
#33
of 59 outputs
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