| Title |
Unraveling the kinetic diversity of microbial 3-dehydroquinate dehydratases of shikimate pathway
|
|---|---|
| Published in |
AMB Express, February 2015
|
| DOI | 10.1186/s13568-014-0087-y |
| Pubmed ID | |
| Authors |
Chang Liu, Yi-Ming Liu, Qing-Lan Sun, Cheng-Ying Jiang, Shuang-Jiang Liu |
| Abstract |
3-Dehydroquinate dehydratase (DHQase) catalyzes the conversion of 3-dehydroquinic acid to 3-dehydroshikimic acid of the shikimate pathway. In this study, 3180 prokaryotic genomes were examined and 459 DHQase sequences were retrieved. Based on sequence analysis and their original hosts, 38 DHQase genes were selected for chemical synthesis. The selected DHQases were translated into new DNA sequences according to the genetic codon usage bias by both Escherichia coli and Corynebacterium glutamicum. The new DNA sequences were customized for synthetic biological applications by adding Biobrick adapters at both ends and by removal of any related restriction endonuclease sites. The customized DHQase genes were successfully expressed in E. coli, and functional DHQases were obtained. Kinetic parameters of Km, kcat, and Vmax of DHQases were determined with a newly established high-throughput method for DHQase activity assay. Results showed that DHQases possessed broad strength of substrate affinities and catalytic capacities. In addition to the DHQase kinetic diversities, this study generated a DHQase library with known catalytic constants that could be applied to design artificial modules of shikimate pathway for metabolic engineering and synthetic biology. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Sweden | 1 | 3% |
| China | 1 | 3% |
| Canada | 1 | 3% |
| Unknown | 32 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 10 | 29% |
| Student > Master | 7 | 20% |
| Researcher | 5 | 14% |
| Student > Ph. D. Student | 3 | 9% |
| Professor | 3 | 9% |
| Other | 4 | 11% |
| Unknown | 3 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 31% |
| Agricultural and Biological Sciences | 8 | 23% |
| Chemistry | 4 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Environmental Science | 1 | 3% |
| Other | 5 | 14% |
| Unknown | 5 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 April 2015.
All research outputs
#20,273,512
of 22,805,349 outputs
Outputs from AMB Express
#968
of 1,234 outputs
Outputs of similar age
#296,609
of 352,584 outputs
Outputs of similar age from AMB Express
#16
of 17 outputs
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