| Title |
Production of transgenic beef cattle rich in n-3 PUFAs by somatic cell nuclear transfer
|
|---|---|
| Published in |
Biotechnology Techniques, May 2015
|
| DOI | 10.1007/s10529-015-1827-z |
| Pubmed ID | |
| Authors |
Gong Cheng, Changzhen Fu, Hong Wang, C. Adoligbe, Shengjuan Wei, Shijun Li, Bijie Jiang, Hongbao Wang, Linsen Zan |
| Abstract |
Omega-3 polyunsaturated fatty acids (n-3 PUFAs) are beneficial to human health. However, the n-3 PUFAs contents of the livestock meat that we consume daily are relatively low. Utilization of transgenic technology to increase n-3 PUFAs contents in livestock may solve this problem. The omega-3 fatty acid desaturase (FAD3), encoded by fat1 gene derived from Caenorhabditis elegans (C. elegans), converts omega-6 polyunsaturated fatty acids (n-6 PUFAs) to n-3 PUFAs. In the study, a plasmid containing the codon-optimized C. elegans fat1 gene (mfat1) was constructed and used to produce transgenic beef cattle by somatic cell nuclear transfer. Fourteen transgenic calves were obtained, and the ratio of n-6 to n-3 PUFAs in the transgenic calves decreased from 5.33: 1 to 0.95: 1 compared with negative controls. Our results demonstrated that the codon-optimized C. elegans mfat1 gene can be functionally expressed in the beef cattle and converts n-6 PUFAs to n-3 PUFAs. |
X Demographics
The data shown below were collected from the profiles of 10 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 10% |
| United Kingdom | 1 | 10% |
| Italy | 1 | 10% |
| Germany | 1 | 10% |
| Netherlands | 1 | 10% |
| Unknown | 5 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 10 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Chile | 1 | 3% |
| Peru | 1 | 3% |
| Unknown | 35 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 16% |
| Student > Ph. D. Student | 5 | 14% |
| Researcher | 4 | 11% |
| Student > Master | 4 | 11% |
| Professor | 2 | 5% |
| Other | 8 | 22% |
| Unknown | 8 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 32% |
| Biochemistry, Genetics and Molecular Biology | 5 | 14% |
| Veterinary Science and Veterinary Medicine | 4 | 11% |
| Environmental Science | 2 | 5% |
| Medicine and Dentistry | 2 | 5% |
| Other | 5 | 14% |
| Unknown | 7 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 104. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 May 2015.
All research outputs
#405,230
of 25,373,627 outputs
Outputs from Biotechnology Techniques
#5
of 2,762 outputs
Outputs of similar age
#4,372
of 278,620 outputs
Outputs of similar age from Biotechnology Techniques
#2
of 30 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,762 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 278,620 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 30 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.