| Title |
Expression of recombinant HBD3 protein that reduces Mycobacterial infection capacity
|
|---|---|
| Published in |
AMB Express, March 2018
|
| DOI | 10.1186/s13568-018-0573-8 |
| Pubmed ID | |
| Authors |
Feng Su, Xin Chen, Xin Liu, Guanghui Liu, Yong Zhang |
| Abstract |
Bovine tuberculosis is a disease caused by Mycobacterium bovis (M. bovis) that leads to great economic losses in cattle production. The discovery of a reasonable bioagent to reduce M. bovis infection risk and environment contamination becomes significant and urgent. Previous study reported that human β-defensin-3 (HBD3) participated in Mycobacterial immunity and was recognized as a suitable candidate reagent. However, its minimal inhibitory concentration to M. bovis is not yet reported. In this study, we first purified HBD3 protein by recombinant-DNA technology and prokaryotic expression system. Subsequently, anti-bacterial tests were used to evaluate the basic bioactivity of the protein. Results revealed that recombinant HBD3 (rHBD3) protein inhibits Staphylococcus multiplication but not the host Escherichia coli. The growth curve of M. bovis showed that rHBD3 protein controls the proliferation of M. bovis in 20 μg/ml concentration. In addition, rHBD3 protein-incubated M. bovis exhibited reduced infectivity to alveolar epithelial cells and macrophages. In conclusion, the expression of rHBD3 protein is a potential ideal bio-regent for reducing M. bovis infection. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 42 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 14% |
| Student > Ph. D. Student | 4 | 10% |
| Researcher | 4 | 10% |
| Other | 2 | 5% |
| Student > Master | 2 | 5% |
| Other | 3 | 7% |
| Unknown | 21 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 5 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 10% |
| Agricultural and Biological Sciences | 4 | 10% |
| Biochemistry, Genetics and Molecular Biology | 1 | 2% |
| Business, Management and Accounting | 1 | 2% |
| Other | 6 | 14% |
| Unknown | 21 | 50% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 March 2018.
All research outputs
#18,591,506
of 23,028,364 outputs
Outputs from AMB Express
#805
of 1,241 outputs
Outputs of similar age
#258,108
of 332,278 outputs
Outputs of similar age from AMB Express
#39
of 60 outputs
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So far Altmetric has tracked 1,241 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 21st percentile – i.e., 21% of its peers scored the same or lower than it.
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We're also able to compare this research output to 60 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.