| Title |
Balanced trafficking between the ER and the Golgi apparatus increases protein secretion in yeast
|
|---|---|
| Published in |
AMB Express, March 2018
|
| DOI | 10.1186/s13568-018-0571-x |
| Pubmed ID | |
| Authors |
Jichen Bao, Mingtao Huang, Dina Petranovic, Jens Nielsen |
| Abstract |
The yeast Saccharomyces cerevisiae is widely used as a cell factory to produce recombinant proteins. However, S. cerevisiae naturally secretes only a few proteins, such as invertase and the mating alpha factor, and its secretory capacity is limited. It has been reported that engineering protein anterograde trafficking from the endoplasmic reticulum to the Golgi apparatus by the moderate overexpression of SEC16 could increase recombinant protein secretion in S. cerevisiae. In this study, the retrograde trafficking in a strain with moderate overexpression of SEC16 was engineered by overexpression of ADP-ribosylation factor GTP activating proteins, Gcs1p and Glo3p, which are involved in the process of COPI-coated vesicle formation. Engineering the retrograde trafficking increased the secretion of α-amylase but did not induce production of reactive oxygen species. An expanded ER membrane was detected in both the GCS1 and GLO3 overexpression strains. Physiological characterizations during batch fermentation showed that GLO3 overexpression had better effect on recombinant protein secretion than GCS1 overexpression. Additionally, the GLO3 overexpression strain had higher secretion of two other recombinant proteins, endoglucanase I from Trichoderma reesei and glucan-1,4-α-glucosidase from Rhizopus oryzae, indicating overexpression of GLO3 in a SEC16 moderate overexpression strain might be a general strategy for improving production of secreted proteins by yeast. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 61 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 14 | 23% |
| Student > Ph. D. Student | 5 | 8% |
| Student > Bachelor | 5 | 8% |
| Professor | 3 | 5% |
| Professor > Associate Professor | 3 | 5% |
| Other | 9 | 15% |
| Unknown | 22 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 16 | 26% |
| Agricultural and Biological Sciences | 11 | 18% |
| Engineering | 5 | 8% |
| Environmental Science | 1 | 2% |
| Chemical Engineering | 1 | 2% |
| Other | 2 | 3% |
| Unknown | 25 | 41% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 March 2018.
All research outputs
#20,468,008
of 23,026,672 outputs
Outputs from AMB Express
#974
of 1,241 outputs
Outputs of similar age
#293,923
of 332,696 outputs
Outputs of similar age from AMB Express
#46
of 55 outputs
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