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Characterization of Spleen Transcriptome of Schizothorax prenanti during Aeromonas hydrophila Infection

Overview of attention for article published in Marine Biotechnology, March 2018
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Title
Characterization of Spleen Transcriptome of Schizothorax prenanti during Aeromonas hydrophila Infection
Published in
Marine Biotechnology, March 2018
DOI 10.1007/s10126-018-9801-0
Pubmed ID
Authors

Hua Ye, Shijun Xiao, Xiaoqing Wang, Zhiyong Wang, Zhengshi Zhang, Chengke Zhu, Bingjie Hu, Changhuan Lv, Shuming Zheng, Hui Luo

Abstract

Schizothorax prenanti (S. prenanti) is an indigenous fish species and is popularly cultured in southwestern China. In recent years, intensive farming of S. prenanti and water quality deterioration has increased the susceptibility of this fish to various pathogens, including Aeromonas hydrophila (A. hydrophila), which has caused severe damage to S. prenanti production. However, the understanding of molecular immune response of S. prenanti to A. hydrophila infection is still lacking. In order to better comprehend the S. prenanti time series immune response process against A. hydrophila, we conducted the first transcriptomic comparison in S. prenanti spleen at 4, 24, and 48 h after the infection challenge of A. hydrophila against their control counterparts. In total, 628 million clean reads were obtained from 18 libraries and assembled into 262,745 transcripts. After eliminating sequence redundancy, 69,373 unigenes with an average length of 1476 bp were obtained. Comparative analysis revealed 1890 unigenes with significantly differential expression, including 172, 455, 589 upregulated and 27, 676, 551 unigenes downregulated genes for 4, 24, and 48 h post-infection, respectively. Differentially expressed genes (DEGs) were validated using qPCR for 15 randomly selected genes. Enrichment and pathway analysis of DEGs was carried out to understand the functions of the immune-related genes. Our results revealed that many important functional genes relating to complement and coagulation cascades, chemokine signaling pathway, toll-like receptor signaling pathway, NOD-like receptor signaling pathway and leukocyte transendothelial migration were regulated during the infection of A. hydrophila, and the expression of those genes reflected the transcriptome profiles during the challenging stages.

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Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 33%
Lecturer 2 13%
Researcher 2 13%
Student > Bachelor 1 7%
Student > Doctoral Student 1 7%
Other 2 13%
Unknown 2 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 53%
Biochemistry, Genetics and Molecular Biology 1 7%
Veterinary Science and Veterinary Medicine 1 7%
Social Sciences 1 7%
Materials Science 1 7%
Other 0 0%
Unknown 3 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 September 2018.
All research outputs
#21,158,537
of 25,988,468 outputs
Outputs from Marine Biotechnology
#275
of 328 outputs
Outputs of similar age
#275,670
of 351,911 outputs
Outputs of similar age from Marine Biotechnology
#1
of 1 outputs
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