| Title |
Enhanced 2-keto-l-gulonic acid production by applying l-sorbose-tolerant helper strain in the co-culture system
|
|---|---|
| Published in |
AMB Express, February 2018
|
| DOI | 10.1186/s13568-018-0562-y |
| Pubmed ID | |
| Authors |
Mandlaa, Ziyu Sun, Ruigang Wang, Xiaodong Han, Hui Xu, Weichao Yang |
| Abstract |
2-Keto-L-gulonic acid (the precursor of vitamin C) is bio-converted from L-sorbose by mixed fermentation of Ketogulonicigenium vulgare and a helper strain. The helper strain promotes the conversion of 2-KLG by enhancing the growth of K. vulgare, but its growth is greatly inhibited by high concentration of L-sorbose, which consequently influence the 2-KLG production. The aim of this study is to obtain L-sorbose-tolerant helper strain (LHS) by experimental evolution for reduced L-sorbose-inhibition-effect and enhanced 2-KLG productivity in high concentration of L-sorbose. After three steps screening by using our devised screening strategy, three strains (i.e., Bc 21, Bc 47, Bc 50) with high resistance to high concentration of L-sorbose were obtained. The fermentation tests by co-culturing Bc 21 and K. vulgare 418 showed that the production of 2-KLG was increased by 17.9% in 11% L-sorbose medium than that in 8% after 55 h of fermentation and the conversion rate was 89.5%. The results suggested that Bc 21 could be an ideal helper strain for 2-KLG production under high concentration of L-sorbose and demonstrated the feasibility of using experimental evolution to breed LHS for vitamin C production. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 23% |
| Student > Master | 2 | 15% |
| Researcher | 2 | 15% |
| Lecturer | 1 | 8% |
| Student > Postgraduate | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 4 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 31% |
| Agricultural and Biological Sciences | 2 | 15% |
| Environmental Science | 1 | 8% |
| Unknown | 6 | 46% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 February 2018.
All research outputs
#15,493,741
of 23,025,074 outputs
Outputs from AMB Express
#447
of 1,241 outputs
Outputs of similar age
#211,234
of 330,534 outputs
Outputs of similar age from AMB Express
#13
of 50 outputs
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