Title |
Analysis of APC mutation in human ameloblastoma and clinical significance
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Published in |
SpringerPlus, March 2016
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DOI | 10.1186/s40064-016-1904-3 |
Pubmed ID | |
Authors |
Ning Li, Bing Liu, Chengguang Sui, Youhong Jiang |
Abstract |
As a highly conserved signaling pathway, Wnt/β-catenin signal transduction pathway plays an important role in many processes. Either in the occurrence or development of tumor, activation of this pathway takes an important place. APC inhibits Wnt/β-catenin pathway to regulate cell proliferation and differentiation. This study aimed to investigate the function of cancer suppressor gene. PCR amplification and sequencing method was used to analyze APC mutations of human clinical specimens. The pathological specimens were collected for PCR and clear electrophoretic bands were obtained after electrophoresis. The gene sequence obtained after purification and sequencing analysis was compared with the known APC gene sequence (NM_000038.5). Base mutations at APC 1543 (T → C), APC-4564 (G → A), APC-5353 (T → G), APC-5550 (T → A) and APC-5969 (G → A) locus existed in 22 (27.5 %), 12 (15 %), 5 (6.25 %), 13 (16.25 %) and 12 patients (15 %), respectively. Gene mutations existed in ameloblastoma, and the mutation loci were 1543 locus (T → C), 4564 locus (G → A), 5353 locus (T → G), 5550 locus (T → A) and 5969 locus (G → A) 15 %, respectively. APC mutation plays a certain role in monitoring the tumor malignant degree as it may indicate the transition process of ameloblastoma malignant phenotype. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Other | 1 | 10% |
Professor | 1 | 10% |
Student > Ph. D. Student | 1 | 10% |
Student > Master | 1 | 10% |
Researcher | 1 | 10% |
Other | 1 | 10% |
Unknown | 4 | 40% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 20% |
Agricultural and Biological Sciences | 1 | 10% |
Biochemistry, Genetics and Molecular Biology | 1 | 10% |
Immunology and Microbiology | 1 | 10% |
Chemistry | 1 | 10% |
Other | 0 | 0% |
Unknown | 4 | 40% |