| Title |
Lignin peroxidase mediated silver nanoparticle synthesis in Acinetobacter sp.
|
|---|---|
| Published in |
AMB Express, December 2017
|
| DOI | 10.1186/s13568-017-0528-5 |
| Pubmed ID | |
| Authors |
Richa Singh, Utkarsha U. Shedbalkar, Shradhda B. Nadhe, Sweety A. Wadhwani, Balu A. Chopade |
| Abstract |
Metals present in environment render the bacteria to attain certain resistance machinery to survive, one of which is transformation of metal ions to nano forms. Various enzymes and proteins have been suggested to play significant role in synthesis of silver nanoparticles (AgNPs) in bacteria. In present study, we have purified lignin peroxidase from secreted enzyme extract of Acinetobacter sp. employing diethyl aminoethyl cellulose ion exchange and Biogel P-150 gel filtration column chromatography. The purified fraction has a specific activity of 1.571 U/mg with substrate n-propanol and 6.5-fold purification. The tetrameric enzyme, with molecular weight of 99 kDa, consisted of dimers of two polypetides of 23.9 and 24.6 kDa as revealed by native and SDS-PAGE. On exposure to purified enzyme, spherical polydispersed AgNPs of ~ 50 nm were obtained as observed under transmission electron microscope. Optimum activity of the purified enzyme was obtained at pH 2 and 60 °C with n-propanol as substrate. This is the first report describing the reduction of extracellular silver ions by lignin peroxidase purified from Acinetobacter sp. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 38 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 18% |
| Student > Master | 6 | 16% |
| Student > Bachelor | 6 | 16% |
| Professor > Associate Professor | 3 | 8% |
| Student > Doctoral Student | 2 | 5% |
| Other | 10 | 26% |
| Unknown | 4 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 21% |
| Agricultural and Biological Sciences | 6 | 16% |
| Chemical Engineering | 3 | 8% |
| Chemistry | 3 | 8% |
| Immunology and Microbiology | 3 | 8% |
| Other | 8 | 21% |
| Unknown | 7 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 December 2017.
All research outputs
#20,456,235
of 23,012,811 outputs
Outputs from AMB Express
#974
of 1,241 outputs
Outputs of similar age
#376,540
of 440,922 outputs
Outputs of similar age from AMB Express
#44
of 45 outputs
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