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Lignin peroxidase mediated silver nanoparticle synthesis in Acinetobacter sp.

Overview of attention for article published in AMB Express, December 2017
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Title
Lignin peroxidase mediated silver nanoparticle synthesis in Acinetobacter sp.
Published in
AMB Express, December 2017
DOI 10.1186/s13568-017-0528-5
Pubmed ID
Authors

Richa Singh, Utkarsha U. Shedbalkar, Shradhda B. Nadhe, Sweety A. Wadhwani, Balu A. Chopade

Abstract

Metals present in environment render the bacteria to attain certain resistance machinery to survive, one of which is transformation of metal ions to nano forms. Various enzymes and proteins have been suggested to play significant role in synthesis of silver nanoparticles (AgNPs) in bacteria. In present study, we have purified lignin peroxidase from secreted enzyme extract of Acinetobacter sp. employing diethyl aminoethyl cellulose ion exchange and Biogel P-150 gel filtration column chromatography. The purified fraction has a specific activity of 1.571 U/mg with substrate n-propanol and 6.5-fold purification. The tetrameric enzyme, with molecular weight of 99 kDa, consisted of dimers of two polypetides of 23.9 and 24.6 kDa as revealed by native and SDS-PAGE. On exposure to purified enzyme, spherical polydispersed AgNPs of ~ 50 nm were obtained as observed under transmission electron microscope. Optimum activity of the purified enzyme was obtained at pH 2 and 60 °C with n-propanol as substrate. This is the first report describing the reduction of extracellular silver ions by lignin peroxidase purified from Acinetobacter sp.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 42 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 17%
Student > Ph. D. Student 7 17%
Student > Bachelor 6 14%
Professor 3 7%
Professor > Associate Professor 3 7%
Other 10 24%
Unknown 6 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 19%
Agricultural and Biological Sciences 6 14%
Immunology and Microbiology 4 10%
Chemical Engineering 3 7%
Chemistry 3 7%
Other 9 21%
Unknown 9 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 December 2017.
All research outputs
#20,456,235
of 23,012,811 outputs
Outputs from AMB Express
#974
of 1,241 outputs
Outputs of similar age
#376,540
of 440,922 outputs
Outputs of similar age from AMB Express
#44
of 45 outputs
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So far Altmetric has tracked 1,241 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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