| Title |
Role of chaperones and ATP synthase in DNA gyrase reactivation in Escherichia coli stationary-phase cells after nutrient addition
|
|---|---|
| Published in |
SpringerPlus, November 2014
|
| DOI | 10.1186/2193-1801-3-656 |
| Pubmed ID | |
| Authors |
Alejandra Gutiérrez-Estrada, Jesús Ramírez-Santos, María del Carmen Gómez-Eichelmann |
| Abstract |
Escherichia coli stationary-phase (SP) cells contain relaxed DNA molecules and recover DNA supercoiling once nutrients become available. In these cells, the reactivation of DNA gyrase, which is a DNA topoisomerase type IIA enzyme, is responsible for the recovery of DNA supercoiling. The results presented in this study show that DNA gyrase reactivation does not require cellular chaperones or polyphosphate. Glucose addition to SP cells induced a slow recovery of DNA supercoiling, whereas resveratrol, which is an inhibitor of ATP synthase, inhibited the enzyme reactivation. These results suggest that DNA gyrase, which is an ATP-dependent enzyme, remains soluble in SP cells, and that its reactivation occurs primarily due to a rapid increase in the cellular ATP concentration. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 17% |
| Student > Master | 3 | 17% |
| Student > Doctoral Student | 2 | 11% |
| Researcher | 2 | 11% |
| Professor > Associate Professor | 2 | 11% |
| Other | 5 | 28% |
| Unknown | 1 | 6% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 44% |
| Biochemistry, Genetics and Molecular Biology | 6 | 33% |
| Chemistry | 2 | 11% |
| Social Sciences | 1 | 6% |
| Unknown | 1 | 6% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 December 2014.
All research outputs
#16,688,979
of 24,546,092 outputs
Outputs from SpringerPlus
#954
of 1,861 outputs
Outputs of similar age
#158,704
of 268,178 outputs
Outputs of similar age from SpringerPlus
#58
of 97 outputs
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