Title |
Comparison of the adjuvanticity of two adjuvant formulations containing de-O-acylated lipooligosaccharide on Japanese encephalitis vaccine in mice
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Published in |
Archives of Pharmacal Research, November 2017
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DOI | 10.1007/s12272-017-0985-z |
Pubmed ID | |
Authors |
Ara Ko, Seo Ri Wui, Ji In Ryu, Hien Thi Thu Do, Yeon Jeong Lee, Soo Jeong Lim, Inmoo Rhee, Dae Im Jung, Jin-ah Park, Jung-ah Choi, Man Ki Song, Na Gyong Lee |
Abstract |
Adjuvants are essential vaccine components used to enhance, accelerate, and/or prolong adaptive immunity against specific vaccine antigens. In this study, we compared the adjuvanticity of two adjuvant formulations containing de-O-acylated lipooligosaccharide (dLOS), a toll-like receptor 4 agonist, on the Japanese encephalitis (JE) vaccine in mice. Mice were immunized once or twice at a two-week interval with inactivated JE vaccine in the absence or presence of adjuvant. We found that both the alum- and the liposome-based formulation induced significantly faster and higher serum IgG antibody responses as compared with the non-adjuvanted vaccine after either one or two immunizations. The antibody titers of the mouse immune sera correlated with 50% plaque reduction neutralization test (PRNT50) antibody titers. In addition, the dLOS/liposome formulation was more effective in inducing a Th1-type immune response than the dLOS/alum formulation, as suggested by a strong antigen-specific interferon (IFN)-γ response. Based on these results, we suggest that both alum- and liposome-based adjuvant formulations containing dLOS may be used for the development of JE vaccines with improved immunogenicity. |
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Unknown | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
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Unknown | 7 | 100% |
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Student > Ph. D. Student | 3 | 43% |
Professor | 1 | 14% |
Student > Bachelor | 1 | 14% |
Unknown | 2 | 29% |
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Chemistry | 2 | 29% |
Immunology and Microbiology | 2 | 29% |
Biochemistry, Genetics and Molecular Biology | 1 | 14% |
Unknown | 2 | 29% |