| Title |
Extraction and characterization of keratin from bovine hoof: A potential material for biomedical applications
|
|---|---|
| Published in |
SpringerPlus, October 2014
|
| DOI | 10.1186/2193-1801-3-596 |
| Pubmed ID | |
| Authors |
Prachi Kakkar, Balaraman Madhan, Ganesh Shanmugam |
| Abstract |
Keratin from the hoof is a less explored source for making valuable products. In this paper we present the extraction of pure keratin from bovine hooves and characterized them to better address the possible exploitation of this bio-resource as an alternative material for tissue engineering applications. The keratin protein from the pulverized hooves was extracted by reduction, which was observed to be pure, and two polypeptide chains of molecular weight in the range of 45-50 and 55-60 KDa were determined using SDS-PAGE assay. FTIR analysis complementing circular dichroism (CD) data, established that hoof keratin predominantly adopted α-helical conformation with admixture of β-sheet. The keratin was shown to have appreciably high denaturation temperature (215°C) as indicated by differential scanning calorimetric (DSC) analysis. Thermogravimetric analysis (TGA) also showed the retention of 50% of the original weight of the sample even at a temperature of 346°C. The keratin from the hoof had been observed to be biocompatible when analyzed with MTT assay using fibroblast cells, showing more than 90% cell viability. Hence, hoof keratin would be useful for high value biomedical applications. |
Mendeley readers
The data shown below were compiled from readership statistics for 150 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 150 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 24 | 16% |
| Student > Bachelor | 22 | 15% |
| Researcher | 17 | 11% |
| Student > Master | 15 | 10% |
| Student > Doctoral Student | 11 | 7% |
| Other | 16 | 11% |
| Unknown | 45 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 20 | 13% |
| Biochemistry, Genetics and Molecular Biology | 17 | 11% |
| Chemistry | 13 | 9% |
| Agricultural and Biological Sciences | 11 | 7% |
| Materials Science | 8 | 5% |
| Other | 30 | 20% |
| Unknown | 51 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 November 2014.
All research outputs
#20,242,136
of 22,769,322 outputs
Outputs from SpringerPlus
#1,461
of 1,852 outputs
Outputs of similar age
#213,557
of 255,612 outputs
Outputs of similar age from SpringerPlus
#83
of 97 outputs
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We're also able to compare this research output to 97 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.