Title |
Assessment of bacterial diversity in the cattle tick Rhipicephalus (Boophilus) microplusthrough tag-encoded pyrosequencing
|
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Published in |
BMC Microbiology, January 2011
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DOI | 10.1186/1471-2180-11-6 |
Pubmed ID | |
Authors |
Renato Andreotti, Adalberto A Pérez de León, Scot E Dowd, Felix D Guerrero, Kylie G Bendele, Glen A Scoles |
Abstract |
Ticks are regarded as the most relevant vectors of disease-causing pathogens in domestic and wild animals. The cattle tick, Rhipicephalus (Boophilus) microplus, hinders livestock production in tropical and subtropical parts of the world where it is endemic. Tick microbiomes remain largely unexplored. The objective of this study was to explore the R. microplus microbiome by applying the bacterial 16S tag-encoded FLX-titanium amplicon pyrosequencing (bTEFAP) technique to characterize its bacterial diversity. Pyrosequencing was performed on adult males and females, eggs, and gut and ovary tissues from adult females derived from samples of R. microplus collected during outbreaks in southern Texas. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 5 | 2% |
Brazil | 2 | <1% |
France | 2 | <1% |
Italy | 1 | <1% |
United Kingdom | 1 | <1% |
New Zealand | 1 | <1% |
Singapore | 1 | <1% |
India | 1 | <1% |
Denmark | 1 | <1% |
Other | 3 | 1% |
Unknown | 282 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 63 | 21% |
Student > Ph. D. Student | 57 | 19% |
Student > Master | 40 | 13% |
Student > Doctoral Student | 20 | 7% |
Student > Bachelor | 20 | 7% |
Other | 44 | 15% |
Unknown | 56 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 137 | 46% |
Biochemistry, Genetics and Molecular Biology | 34 | 11% |
Veterinary Science and Veterinary Medicine | 20 | 7% |
Environmental Science | 12 | 4% |
Medicine and Dentistry | 10 | 3% |
Other | 22 | 7% |
Unknown | 65 | 22% |