| Title |
Optimization of culture conditions for rapid clinical‐scale expansion of human umbilical cord blood‐derived mesenchymal stem cells
|
|---|---|
| Published in |
Clinical and Translational Medicine, October 2017
|
| DOI | 10.1186/s40169-017-0168-z |
| Pubmed ID | |
| Authors |
Wankyu Choi, Soon-Jae Kwon, Hye Jin Jin, Sang Young Jeong, Soo Jin Choi, Wonil Oh, Yoon Sun Yang, Hong Bae Jeon, Eun Su Jeon |
| Abstract |
Mesenchymal stem cells (MSCs) have broad-spectrum therapeutic effects in various diseases, and thus have many clinical applications. However, it is difficult to produce sufficient numbers of MSCs for clinical use, and improved culture systems are required. Here, we report the effects of calcium (Ca(2+)) and hypoxia on the proliferation of human umbilical cord blood-derived MSCs (hUCB-MSCs). In addition, we determined the optimal conditions of these two factors for the large-scale culture of hUCB-MSCs. hUCB-MSCs were maintained under hypoxic conditions (3% O2) with 1.8 mM Ca(2+) during long-term culture, and their proliferation was evaluated. To characterize the underlying mechanisms, the effects on hypoxia-inducible factor (HIF)-1α and the extracellular signal-regulated kinase (ERK) signaling pathways were investigated. The therapeutic effects in a mouse emphysema model were analyzed and compared with those of naive MSCs. The proliferation of Ca(2+)/hypoxia-treated hUCB-MSCs was increased compared with that observed using either calcium or hypoxia culture alone, without loss of stem cell marker expression or differentiation ability. The enhancement of the proliferation capacity of hUCB-MSCs by the synergistic effects of Ca(2+) and hypoxia was dependent on the expression of HIF-1α and the ERK signaling pathway. The proliferation of Ca(2+)/hypoxia-treated hUCB-MSCs resulted in a delayed senescence phenotype and increased the expression levels of stemness genes such as Oct4 and Nanog compared to those observed in conventional culture conditions. In addition, Ca(2+)/hypoxia-treated MSCs transplantation in the mouse emphysema model showed the same therapeutic effects as observed with naive MSCs. These findings suggest that a Ca(2+)/hypoxia-based expansion system has applications for the large-scale production of MSCs for therapeutic purposes. |
X Demographics
The data shown below were collected from the profiles of 9 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 22% |
| Ireland | 1 | 11% |
| Unknown | 6 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 8 | 89% |
| Science communicators (journalists, bloggers, editors) | 1 | 11% |
Mendeley readers
The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 31 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 19% |
| Student > Bachelor | 4 | 13% |
| Student > Master | 4 | 13% |
| Student > Ph. D. Student | 3 | 10% |
| Student > Doctoral Student | 2 | 6% |
| Other | 4 | 13% |
| Unknown | 8 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 32% |
| Agricultural and Biological Sciences | 5 | 16% |
| Medicine and Dentistry | 3 | 10% |
| Chemical Engineering | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 10 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 October 2023.
All research outputs
#3,059,016
of 25,756,531 outputs
Outputs from Clinical and Translational Medicine
#117
of 1,080 outputs
Outputs of similar age
#54,622
of 334,781 outputs
Outputs of similar age from Clinical and Translational Medicine
#1
of 10 outputs
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