| Title |
Glucocorticoids promote neural progenitor cell proliferation derived from human induced pluripotent stem cells
|
|---|---|
| Published in |
SpringerPlus, September 2014
|
| DOI | 10.1186/2193-1801-3-527 |
| Pubmed ID | |
| Authors |
Eiichi Ninomiya, Taeka Hattori, Masashi Toyoda, Akihiro Umezawa, Takashi Hamazaki, Haruo Shintaku |
| Abstract |
Glucocorticoids (GCs) are frequently used for treating and preventing chronic lung disease and circulatory dysfunction in premature infants. However, there is growing concern about the detrimental effects of systemic GC administration on neurodevelopment. The first choice of GCs to minimize the adverse effects on the developing brain is still under debate. We investigated the effect of commonly used GCs such as dexamethasone (DEX), betamethasone (BET) and hydrocortisone (HDC) on the proliferation of human-induced pluripotent stem cell (iPSC)-derived neuronal progenitor cells (NPCs). In this study, NPCs were treated with various concentrations of GCs and subjected to cell proliferation assays. Furthermore, we quantified the number of microtubule-associated protein 2 (MAP2) positive neurons in NPCs by immunostaining. All GCs promoted NPC proliferation in a dose-dependent manner. We also confirmed that MAP2-positive neurons in NPCs increased upon GC treatment. However, differential effects of GCs on MAP2 positive neurons were observed when we treated NPCs with H2O2. The total numbers of NPCs increased upon any GC treatment even under oxidative conditions but the numbers of MAP2 positive neurons increased only by HDC treatment. GCs promoted human iPSCsâ€"derived NPC proliferation and the differential effects of GCs became apparent under oxidative stress. Our results may support HDC as the preferred choice over DEX and BET to prevent adverse effects on the developing human brain. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 81 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 1% |
| United States | 1 | 1% |
| Unknown | 79 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 22 | 27% |
| Other | 21 | 26% |
| Student > Ph. D. Student | 12 | 15% |
| Student > Bachelor | 8 | 10% |
| Student > Master | 3 | 4% |
| Other | 9 | 11% |
| Unknown | 6 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 41 | 51% |
| Medicine and Dentistry | 7 | 9% |
| Biochemistry, Genetics and Molecular Biology | 7 | 9% |
| Neuroscience | 6 | 7% |
| Engineering | 3 | 4% |
| Other | 8 | 10% |
| Unknown | 9 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 September 2014.
All research outputs
#15,305,567
of 22,763,032 outputs
Outputs from SpringerPlus
#931
of 1,852 outputs
Outputs of similar age
#143,104
of 246,452 outputs
Outputs of similar age from SpringerPlus
#55
of 105 outputs
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So far Altmetric has tracked 1,852 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
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We're also able to compare this research output to 105 others from the same source and published within six weeks on either side of this one. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.