| Title |
Identification of a gene, FMP21, whose expression levels are involved in thermotolerance in Saccharomyces cerevisiae
|
|---|---|
| Published in |
AMB Express, August 2014
|
| DOI | 10.1186/s13568-014-0067-2 |
| Pubmed ID | |
| Authors |
Toshihide Nakamura, Mami Yamamoto, Katsuichi Saito, Akira Ando, Jun Shima |
| Abstract |
Elucidation of the mechanism of high temperature tolerance in yeasts is important for the molecular breeding of high temperature-tolerant yeasts that can be used in bioethanol production. We identified genes whose expression is correlated with the degree of thermotolerance in Saccharomyces cerevisiae by DNA microarray analysis. Gene expression profiles of three S. cerevisiae strains showing different levels of thermotolerance were compared, and we chose three of them as candidate genes. Among these genes, FMP21 was investigated as a thermotolerance-related gene in S. cerevisiae by comparing the growth at high temperature with the gene expression in eight strains. The expression ratio of FMP21 at 37°C was correlated with the doubling time ratio at a coefficient of determination of 0.787. The potential involvement of the Fmp21 in the thermotolerance of yeasts was evaluated. The FMP21 deletion variant showed a decreased respiratory growth rate and increased thermosensitivity. Furthermore, the overexpression of FMP21 improved thermotolerance in yeasts. In conclusion, the function of Fmp21 is important for thermotolerance in yeasts. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 4% |
| Unknown | 25 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 31% |
| Student > Doctoral Student | 4 | 15% |
| Student > Postgraduate | 3 | 12% |
| Student > Ph. D. Student | 3 | 12% |
| Lecturer | 2 | 8% |
| Other | 5 | 19% |
| Unknown | 1 | 4% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 50% |
| Biochemistry, Genetics and Molecular Biology | 7 | 27% |
| Engineering | 2 | 8% |
| Veterinary Science and Veterinary Medicine | 1 | 4% |
| Materials Science | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 1 | 4% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 August 2014.
All research outputs
#20,235,415
of 22,761,738 outputs
Outputs from AMB Express
#965
of 1,231 outputs
Outputs of similar age
#197,659
of 235,668 outputs
Outputs of similar age from AMB Express
#12
of 20 outputs
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