| Title |
Multiplex PCR assay for identification of commonly used disarmed Agrobacterium tumefaciens strains
|
|---|---|
| Published in |
SpringerPlus, July 2014
|
| DOI | 10.1186/2193-1801-3-358 |
| Pubmed ID | |
| Authors |
Farah Deeba, Muhammad Zeeshan Hyder, Shahzad Hussain Shah, Syed Muhammad Saqlan Naqvi |
| Abstract |
The success of Agrobacterium mediated plant transformation depends to a certain extent on appropriate selection of the A. tumefaciens strain for a particular plant species. Many stages in a plant transformation procedure are prone to bacterial contamination with similar antibiotic resistance that may compromise the identity of the A. tumefaciens strain used, in turn adversely affecting success of a transformation experiment. Different primer sets were designed to exploit genetic differences among different strains of A. tumefaciens which are commonly used for plant genetic transformation, to identity confirmation as well as to distinguish them from one another. The primer sets Ach5FtsZ-F/R specific for Ach5 and C58GlyA-F/R specific for C58 were designed on chromosomal DNA while primer sets pTiBo542-F/R and nptI-F/R specific for plasmid pTiBo542 are capable to identify and distinguish these strains from one another. These primer sets when used simultaneously in multiplex PCR, produce a pattern which uniquely identifies all these strains and distinguishes them except for GV3101 and C58C1, which can further be distinguished from each other by rifampicin screening. The multiplex PCR assay and primers being reported here serve as a valuable tool in determining the identity of A. tumefaciens strains at any stage of plant transformation procedure. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 75 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Philippines | 1 | 1% |
| Unknown | 74 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 21% |
| Researcher | 13 | 17% |
| Student > Master | 12 | 16% |
| Student > Bachelor | 5 | 7% |
| Student > Doctoral Student | 3 | 4% |
| Other | 8 | 11% |
| Unknown | 18 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 33 | 44% |
| Biochemistry, Genetics and Molecular Biology | 18 | 24% |
| Unspecified | 3 | 4% |
| Environmental Science | 2 | 3% |
| Nursing and Health Professions | 1 | 1% |
| Other | 2 | 3% |
| Unknown | 16 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 August 2014.
All research outputs
#18,376,056
of 22,760,687 outputs
Outputs from SpringerPlus
#1,261
of 1,852 outputs
Outputs of similar age
#162,494
of 226,959 outputs
Outputs of similar age from SpringerPlus
#73
of 94 outputs
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