| Title |
Cloning and expression of Aspergillus flavus urate oxidase in Pichia pastoris
|
|---|---|
| Published in |
SpringerPlus, July 2014
|
| DOI | 10.1186/2193-1801-3-395 |
| Pubmed ID | |
| Authors |
Ramin Fazel, Najmeh Zarei, Nasser Ghaemi, Mohammad Mehdi Namvaran, Somayeh Enayati, Esmat Mirabzadeh Ardakani, Mohammad Azizi, Vahid Khalaj |
| Abstract |
Urate oxidase is an important enzyme with therapeutic and diagnostic applications. Rasburicase is a recombinant urate oxidase enzyme approved by FDA to use in the treatment of hyperuricemia conditions. Various hosts such as Saccharomyces cerevisiae, Hansenula polymorpha and Escherichia coli have been used to express the enzyme. Today, Pichia pastoris is considered as an important host for heterologous protein expression since it has beneficial characteristics such as strong promoters, simple scale up, post translational modifications, high cell density cultivation and simple genetic manipulation. In this study, Aspergillus flavus urate oxidase gene was cloned in pPICZαA expression vector and expressed in P. pastoris. The recombinant urate oxidase was expressed in secretory form and was confirmed through RT-PCR, SDS-PAGE analysis and western blotting. The enzyme activity was determined using a colorimetric assay. A production yield of 0.43 U/ml of culture supernatant was obtained. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 51 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 25% |
| Researcher | 8 | 16% |
| Student > Master | 7 | 14% |
| Student > Bachelor | 6 | 12% |
| Professor > Associate Professor | 3 | 6% |
| Other | 6 | 12% |
| Unknown | 8 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 17 | 33% |
| Agricultural and Biological Sciences | 14 | 27% |
| Medicine and Dentistry | 4 | 8% |
| Immunology and Microbiology | 2 | 4% |
| Business, Management and Accounting | 1 | 2% |
| Other | 5 | 10% |
| Unknown | 8 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 August 2014.
All research outputs
#20,233,547
of 22,759,618 outputs
Outputs from SpringerPlus
#1,461
of 1,852 outputs
Outputs of similar age
#192,512
of 228,682 outputs
Outputs of similar age from SpringerPlus
#83
of 94 outputs
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