| Title |
Mitigating role of thymoquinone rich fractions from Nigella sativa oil and its constituents, thymoquinone and limonene on lipidemic-oxidative injury in rats
|
|---|---|
| Published in |
SpringerPlus, June 2014
|
| DOI | 10.1186/2193-1801-3-316 |
| Pubmed ID | |
| Authors |
Shafeeque Ahmad, Zafarul H Beg |
| Abstract |
Therapeutic role of Nigella sativa (NS) seed oil fractions, methanolic extract (ME) and volatile oil (VO) and their constituents, thymoquinone (TQ) and limonene (LMN) in relation to lipidemic-oxidative stress in Wistar rats was determined. The total phenolic contents of NS seed oil and their ME and VO extracts were 320.00 ± 3.00, 300.12 ± 0.04 and 288.41 ± 0.01 mg gallic acid equivalents per 100 g of NS oil, respectively. Their Fe(+2) chelating activities were 870.00 ± 2.00, 222.31 ± 5.80 and 38.59 ± 1.43 mg EDTA equivalents per 100 g of NS oil, respectively. These fractions and compounds exhibited strong antioxidant activities against 2,2-diphenyl-1-picryl hydrazyl, 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid, nitric oxide and hydroxyl radicals. Potential antiperoxidative effects of these fractions and compounds were also observed in liposome, and lipidemic-induced lipid peroxidation in atherogenic suspension fed rats, pretreated with 100 mg ME, 20 mg VO, 10 mg pure TQ or 200 mg LMN for 30 days. ME containing ω-6 linoleic acid and palmitic acid natural compounds was highly effective against lipidemic oxidative stress than VO extract possessing thymol and isothymol phenolic natural antioxidant compounds. TQ, principal compound shared to both the extracts. The test fractions and compounds effectively reduced the erythrocyte and liver lipid peroxidation markers, conjugated diene, lipid hydroperoxide and malondialdehyde to near normal levels in the order ME > TQ > VO > LMN, by directly counteracting free radicals as well as suppressing hepatic HMG-CoA reductase activity. Our findings demonstrated that these natural products, preferably ME possess significant antioxidant activities, and may be recommended as new potential sources of natural antioxidants. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Romania | 1 | 2% |
| Unknown | 41 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 19% |
| Student > Bachelor | 6 | 14% |
| Lecturer | 4 | 10% |
| Lecturer > Senior Lecturer | 4 | 10% |
| Professor > Associate Professor | 4 | 10% |
| Other | 14 | 33% |
| Unknown | 2 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 9 | 21% |
| Medicine and Dentistry | 6 | 14% |
| Agricultural and Biological Sciences | 5 | 12% |
| Unspecified | 3 | 7% |
| Biochemistry, Genetics and Molecular Biology | 3 | 7% |
| Other | 6 | 14% |
| Unknown | 10 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 July 2014.
All research outputs
#18,375,064
of 22,758,963 outputs
Outputs from SpringerPlus
#1,261
of 1,852 outputs
Outputs of similar age
#163,781
of 227,907 outputs
Outputs of similar age from SpringerPlus
#58
of 77 outputs
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