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Bioluminescence imaging to track real-time armadillo promoter activity in live Drosophila embryos

Overview of attention for article published in Analytical & Bioanalytical Chemistry, July 2014
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  • Above-average Attention Score compared to outputs of the same age and source (59th percentile)

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Readers on

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14 Mendeley
Title
Bioluminescence imaging to track real-time armadillo promoter activity in live Drosophila embryos
Published in
Analytical & Bioanalytical Chemistry, July 2014
DOI 10.1007/s00216-014-8000-8
Pubmed ID
Authors

Ryutaro Akiyoshi, Taro Kaneuch, Toshiro Aigaki, Hirobumi Suzuki

Abstract

We established a method for bioluminescence imaging (BLI) to track real-time gene expression in live Drosophila embryos. We constructed a transgenesis vector containing multiple cloning sites and enhanced green-emitting luciferase (ELuc; Emerald Luc), a brighter and pH-insensitive luciferase for promoter analysis. To evaluate the utility of BLI using an ELuc reporter together with an optimized microscope system, we visualized the expression pattern of armadillo (arm), a member of the Wnt pathway in Drosophila, throughout embryogenesis. We generated transgenic flies carrying the arm:: ELuc fusion gene, and successfully performed BLI continuously for 22 h in the same embryos. Our study showed, for the first time, that arm::Eluc expression was dramatically increased in the anterior midgut rudiment, myoblasts of the dorsal/lateral musculature, and the posterior spiracle after stage 13, and the cephalic region at stage 17. To further demonstrate the application of our BLI system, we revealed that arm transcriptional activity in embryos was modulated inversely by treatment with ionomycin or 6-bromoindirubin-3-oxime (BIO), an inhibitor and activator of Wnt/β-catenin signaling, respectively. Therefore, our microscopic BLI system is useful for monitoring gene expression in live Drosophila embryos, and for investigating regulatory mechanisms by using chemicals and mutations that might affect expression.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 29%
Unspecified 3 21%
Student > Ph. D. Student 2 14%
Student > Postgraduate 1 7%
Student > Master 1 7%
Other 3 21%
Readers by discipline Count As %
Unspecified 4 29%
Agricultural and Biological Sciences 4 29%
Chemistry 3 21%
Computer Science 1 7%
Biochemistry, Genetics and Molecular Biology 1 7%
Other 1 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 July 2014.
All research outputs
#7,610,658
of 12,230,555 outputs
Outputs from Analytical & Bioanalytical Chemistry
#2,391
of 4,657 outputs
Outputs of similar age
#99,682
of 198,857 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#17
of 52 outputs
Altmetric has tracked 12,230,555 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,657 research outputs from this source. They receive a mean Attention Score of 2.4. This one is in the 48th percentile – i.e., 48% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 198,857 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 52 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.