Title |
The Cytoplasmic Domain of Rat Synaptotagmin I Enhances Synaptic Transmission
|
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Published in |
Cellular and Molecular Neurobiology, March 2014
|
DOI | 10.1007/s10571-014-0040-2 |
Pubmed ID | |
Authors |
Shao-Ying Hua, Ali Syed, Thierry C. Aupérin, Liang Tong |
Abstract |
Synaptotagmin, an integral membrane protein of synaptic vesicles, functions as a calcium sensor in the temporal control of neurotransmitter release. Although synaptotagmin facilitates lipid membrane fusion in biochemical experiments, overexpression of synaptotagmin inhibits neurotransmission. A facilitatory effect of synaptotagmin on synaptic transmission was never observed. To determine whether synaptotagmin may accelerate synaptic transmission in vivo, we injected the cytoplasmic domain of rat synaptotagmin I (CD-syt) into crayfish motor axons and tested the effect of CD-syt on synaptic response. We confirmed that CD-syt accelerates neuromuscular transmission. The injected preparation had larger synaptic potentials with shorter rise time. Experiments with varying calcium concentrations showed that CD-syt increased the maximum synaptic response of the neuromuscular synapses. Further tests on short-term plasticity of neuromuscular synapses revealed that CD-syt increases the release probability of the release-ready vesicles. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 33% |
Professor > Associate Professor | 1 | 17% |
Researcher | 1 | 17% |
Student > Master | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 50% |
Neuroscience | 2 | 33% |
Unknown | 1 | 17% |