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Cell-specific and endothelium-dependent regulations of matrix metalloproteinase-2 in rat aorta

Overview of attention for article published in Basic Research in Cardiology, June 2014
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Title
Cell-specific and endothelium-dependent regulations of matrix metalloproteinase-2 in rat aorta
Published in
Basic Research in Cardiology, June 2014
DOI 10.1007/s00395-014-0419-8
Pubmed ID
Authors

Irakli Kopaliani, Melanie Martin, Birgit Zatschler, Katrin Bortlik, Bianca Müller, Andreas Deussen

Abstract

Chronic activation of angiotensin II (ANGII) and matrix metalloproteinase-2 (MMP-2) during hypertension contributes to increased aortic stiffness. We studied signalling mechanisms employed by ANGII in the regulation of latent (pro-) and active forms of MMP-2 in rat aortic endothelial and smooth muscle cells, along with isolated rat aorta. Using western blotting, we demonstrate that ANGII (1 µmol/L) significantly (P < 0.01) increases pro-MMP-2 protein expression after 8 h not only in endothelial and smooth muscle cells, but also in isolated rat aorta. We demonstrate that ANGII acts via AT1 receptor-activated cell-specific pathways. In endothelial cells, the JNK1/c-jun pathway is activated, whereas in smooth muscle cells, the JAK2/STAT3 pathway. Activation of JAK2/STAT3 pathway in response to ANGII was EGF receptor-dependent. Results obtained in cell culture are in agreement with the results obtained in isolated aorta. However, active MMP-2 was not found under cell culture conditions, whereas in isolated aorta, active MMP-2 was significantly (P < 0.05) increased after stimulation with ANGII, as detected by gelatine zymography. This increase of MMP-2 activity was not inhibited by blocking the pathways we identified to control pro-MMP-2 protein expression, but was abolished in the absence of endothelium. Our findings demonstrate that ANGII regulates pro-MMP-2 protein expression via cell-specific pathways in rat aorta. The endothelium may play an essential role in the activation of pro-MMP-2. These results may lead to new strategies for inhibiting MMP-2 expression and activity in distinct cell types of the aortic wall.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 3 27%
Researcher 3 27%
Student > Ph. D. Student 3 27%
Student > Master 1 9%
Professor 1 9%
Other 0 0%
Readers by discipline Count As %
Medicine and Dentistry 6 55%
Agricultural and Biological Sciences 2 18%
Unspecified 2 18%
Chemistry 1 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 June 2014.
All research outputs
#10,833,297
of 12,220,852 outputs
Outputs from Basic Research in Cardiology
#346
of 404 outputs
Outputs of similar age
#163,981
of 199,501 outputs
Outputs of similar age from Basic Research in Cardiology
#5
of 6 outputs
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So far Altmetric has tracked 404 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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