| Title |
Dynamic Cellular Uptake of Mixed-Monolayer Protected Nanoparticles
|
|---|---|
| Published in |
Biointerphases, February 2012
|
| DOI | 10.1007/s13758-011-0017-3 |
| Pubmed ID | |
| Authors |
Randy P. Carney, Tamara M. Carney, Marie Mueller, Francesco Stellacci |
| Abstract |
Nanoparticles (NPs) are gaining increasing attention for potential application in medicine; consequently, studying their interaction with cells is of central importance. We found that both ligand arrangement and composition on gold nanoparticles play a crucial role in their cellular internalization. In our previous investigation, we showed that 66-34OT nanoparticles coated with stripe-like domains of hydrophobic (octanethiol, OT, 34%) and hydrophilic (11-mercaptoundecane sulfonate, MUS, 66%) ligands permeated through the cellular lipid bilayer via passive diffusion, in addition to endo-/pino-cytosis. Here, we show an analysis of NP internalization by DC2.4, 3T3, and HeLa cells at two temperatures and multiple time points. We study four NPs that differ in their surface structures and ligand compositions and report on their cellular internalization by intracellular fluorescence quantification. Using confocal laser scanning microscopy we have found that all three cell types internalize the 66-34OT NPs more than particles coated only with MUS, or particles coated with a very similar coating but lacking any detectable ligand shell structure, or 'striped' particles but with a different composition (34-66OT) at multiple data points. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Practitioners (doctors, other healthcare professionals) | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 2 | 3% |
| Italy | 1 | 2% |
| Brazil | 1 | 2% |
| United Kingdom | 1 | 2% |
| United States | 1 | 2% |
| Unknown | 54 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 21 | 35% |
| Researcher | 16 | 27% |
| Student > Master | 6 | 10% |
| Student > Doctoral Student | 5 | 8% |
| Professor > Associate Professor | 2 | 3% |
| Other | 5 | 8% |
| Unknown | 5 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 19 | 32% |
| Materials Science | 10 | 17% |
| Agricultural and Biological Sciences | 7 | 12% |
| Biochemistry, Genetics and Molecular Biology | 5 | 8% |
| Physics and Astronomy | 5 | 8% |
| Other | 5 | 8% |
| Unknown | 9 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 15. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 June 2020.
All research outputs
#2,255,544
of 23,947,846 outputs
Outputs from Biointerphases
#22
of 544 outputs
Outputs of similar age
#17,867
of 254,048 outputs
Outputs of similar age from Biointerphases
#1
of 5 outputs
Altmetric has tracked 23,947,846 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 544 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.4. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 254,048 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them