| Title |
Detection of mesenchymal stem cells senescence by prelamin A accumulation at the nuclear level
|
|---|---|
| Published in |
SpringerPlus, August 2016
|
| DOI | 10.1186/s40064-016-3091-7 |
| Pubmed ID | |
| Authors |
Chiara Bellotti, Cristina Capanni, Giovanna Lattanzi, Davide Donati, Enrico Lucarelli, Serena Duchi |
| Abstract |
Human mesenchymal stem cells (MSC), during in vitro expansion, undergo a progressive loss of proliferative potential that leads to the senescent state, associated with a reduction of their "medicinal" properties. This may hampers their efficacy in the treatment of injured tissues. Quality controls on MSC-based cell therapy products should include an assessment of the senescent state. However, a reliable and specific marker is still missing. From studies on lamin-associated disorders, has emerged the correlation between defective lamin A maturation and cellular senescence. Primary cultured hMSC lines (n = 3), were analyzed by immunostaining at different life-span stages for the accumulation of prelamin A, along with other markers of cellular senescence. During culture, cells at the last stage of their life span displayed evident signs of senescence consistent with the positivity of SA-β-gal staining. We also observed a significant increase of prelamin A positive cells. Furthermore, we verified that the cells marked by prelamin A were also positive for p21(Waf1) while negative for Ki67. Overall data support that the detection of prelamin A identifies senescent MSC, providing an easy and reliable tool to be use alone or in combination with known senescence markers to screen MSC before their use in clinical applications. |
Mendeley readers
The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 48 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 14 | 29% |
| Researcher | 11 | 23% |
| Student > Bachelor | 4 | 8% |
| Student > Doctoral Student | 3 | 6% |
| Other | 2 | 4% |
| Other | 4 | 8% |
| Unknown | 10 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 25% |
| Agricultural and Biological Sciences | 12 | 25% |
| Medicine and Dentistry | 8 | 17% |
| Engineering | 3 | 6% |
| Immunology and Microbiology | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 10 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 October 2022.
All research outputs
#3,776,336
of 23,009,818 outputs
Outputs from SpringerPlus
#229
of 1,855 outputs
Outputs of similar age
#64,461
of 339,245 outputs
Outputs of similar age from SpringerPlus
#38
of 207 outputs
Altmetric has tracked 23,009,818 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,855 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 339,245 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 207 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.