| Title |
Artificial oxidative stress-tolerant Corynebacterium glutamicum
|
|---|---|
| Published in |
AMB Express, March 2014
|
| DOI | 10.1186/s13568-014-0015-1 |
| Pubmed ID | |
| Authors |
Joo-Young Lee, Hyo Jung Lee, Jiyoon Seo, Eung-Soo Kim, Heung-Shick Lee, Pil Kim |
| Abstract |
We have reported a transcription profile of an adapted Corynebacterium glutamicum that showed enhanced oxidative stress resistance. To construct an artificial oxidative stress-resistant strain, gene clusters in the β-ketoadipate pathway, which were up-regulated in the adapted strain, were artificially expressed in the wild-type C. glutamicum. The wild-type strain was unable to grow under 2 mM H2O2 containing minimal medium, while the strains expressing pca gene clusters restored growth under the same medium, and the pcaHGBC expression showed the most significant effect among the gene clusters. The expressions of pca gene clusters also enabled the wild-type to increase its resistance against oxidative stressors, such as diamide and cumene hydroperoxide, as well as H2O2. The oxidative stress tolerance of the strain was correlated to the reactive oxygen species (ROS)-scavenging activity of the cell extract. The reason for the enhanced oxidative stress-resistance of C. glutamicum and its applications on the synthetic strain development are discussed. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 27% |
| Student > Master | 6 | 23% |
| Researcher | 4 | 15% |
| Student > Doctoral Student | 4 | 15% |
| Student > Bachelor | 3 | 12% |
| Other | 0 | 0% |
| Unknown | 2 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 35% |
| Biochemistry, Genetics and Molecular Biology | 8 | 31% |
| Engineering | 3 | 12% |
| Chemical Engineering | 2 | 8% |
| Immunology and Microbiology | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 3 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 March 2014.
All research outputs
#20,224,618
of 22,749,166 outputs
Outputs from AMB Express
#965
of 1,229 outputs
Outputs of similar age
#208,830
of 242,903 outputs
Outputs of similar age from AMB Express
#7
of 17 outputs
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