| Title |
Michael hydratase alcohol dehydrogenase or just alcohol dehydrogenase?
|
|---|---|
| Published in |
AMB Express, March 2014
|
| DOI | 10.1186/s13568-014-0030-2 |
| Pubmed ID | |
| Authors |
Verena Resch, Jianfeng Jin, Bi-Shuang Chen, Ulf Hanefeld |
| Abstract |
The Michael hydratase - alcohol dehydrogenase (MhyADH) from Alicycliphilus denitrificans was previously identified as a bi-functional enzyme performing a hydration of α,β-unsaturated ketones and subsequent oxidation of the formed alcohols. The investigations of the bi-functionality were based on a spectrophotometric assay and an activity staining in a native gel of the dehydrogenase. New insights in the recently discovered organocatalytic Michael addition of water led to the conclusion that the previously performed experiments to identify MhyADH as a bi-functional enzyme and their results need to be reconsidered and the reliability of the methodology used needs to be critically evaluated. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 5 | 29% |
| Student > Ph. D. Student | 3 | 18% |
| Student > Bachelor | 2 | 12% |
| Researcher | 2 | 12% |
| Lecturer > Senior Lecturer | 1 | 6% |
| Other | 3 | 18% |
| Unknown | 1 | 6% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 41% |
| Chemistry | 4 | 24% |
| Biochemistry, Genetics and Molecular Biology | 3 | 18% |
| Medicine and Dentistry | 1 | 6% |
| Unknown | 2 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 March 2014.
All research outputs
#20,224,618
of 22,749,166 outputs
Outputs from AMB Express
#965
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Outputs of similar age
#189,771
of 221,161 outputs
Outputs of similar age from AMB Express
#7
of 16 outputs
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