| Title |
Production of soluble eukaryotic recombinant proteins in E. coli is favoured in early log-phase cultures induced at low temperature
|
|---|---|
| Published in |
SpringerPlus, March 2013
|
| DOI | 10.1186/2193-1801-2-89 |
| Pubmed ID | |
| Authors |
Teresa San-Miguel, Pedro Pérez-Bermúdez, Isabel Gavidia |
| Abstract |
Producing recombinant plant proteins expressed in Escherichia coli produce in high yields and in a soluble and functional form can be difficult. Under overexpression conditions, proteins frequently accumulate as insoluble aggregates (inclusion bodies) within the producing bacteria. We evaluated how the initial culture density, temperature and duration of the expression stage affect the production of some eukaryotic enzymes in E. coli. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 446 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 2 | <1% |
| Portugal | 1 | <1% |
| Finland | 1 | <1% |
| Denmark | 1 | <1% |
| Greece | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 439 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 94 | 21% |
| Student > Bachelor | 83 | 19% |
| Student > Master | 68 | 15% |
| Researcher | 34 | 8% |
| Student > Doctoral Student | 25 | 6% |
| Other | 41 | 9% |
| Unknown | 101 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 128 | 29% |
| Biochemistry, Genetics and Molecular Biology | 125 | 28% |
| Chemistry | 24 | 5% |
| Immunology and Microbiology | 18 | 4% |
| Engineering | 10 | 2% |
| Other | 36 | 8% |
| Unknown | 105 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 March 2021.
All research outputs
#3,187,562
of 22,747,498 outputs
Outputs from SpringerPlus
#191
of 1,853 outputs
Outputs of similar age
#27,589
of 195,279 outputs
Outputs of similar age from SpringerPlus
#9
of 128 outputs
Altmetric has tracked 22,747,498 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,853 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 195,279 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 128 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.