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Investigation into the antimicrobial action and mechanism of a novel endogenous peptide β-casein 197 from human milk

Overview of attention for article published in AMB Express, June 2017
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39 Mendeley
Title
Investigation into the antimicrobial action and mechanism of a novel endogenous peptide β-casein 197 from human milk
Published in
AMB Express, June 2017
DOI 10.1186/s13568-017-0409-y
Pubmed ID
Authors

Yanrong Fu, Chenbo Ji, Xiaohui Chen, Xianwei Cui, Xing Wang, Jie Feng, Yun Li, Rui Qin, Xirong Guo

Abstract

A novel endogenous peptide cleaved from 197-213 AA of β-casein, named β-casein 197, was identified by tandem mass spectrometry. β-casein 197 constituted a significant proportion of the peptide content in preterm milk. This study investigated the antibacterial effects and mechanisms against common pathogenic bacteria. Six bacterial strains were selected for this study: Escherichia coli, Staphylococcus aureus, Yersinia enterocolitica, Listeria monocytogenes, Klebsiella pneumonia and Bacillus subtilis. After synthesis, serial twofold dilutions of β-casein 197 were added to select for sensitive bacteria. The disk diffusion method and analysis of bacterial staining were used to identify antibacterial effect, while DNA-binding, scanning electron microscopy and transmission electron microscopy were used to explore antimicrobial mechanisms. Disk diffusion showed that E. coli, S. aureus and Y. enterocolitica were sensitive to the β-casein 197. In addition, live/dead fluorescent staining also confirmed antibacterial effects. Scanning electron and transmission electron microscopy revealed affected extracellular and intracellular structure for three species of bacteria, while a DNA-binding assay showed that the antimicrobial activity did not occur through DNA binding. This study suggests that β-casein 197 has antimicrobial activity against common pathogenic bacteria in newborns with infection. The peptide induced membrane permeabilization but did not bind to genomic DNA. Based on our findings, β-casein 197 has potential clinical value for preventing infections of premature infants.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 39 100%

Demographic breakdown

Readers by professional status Count As %
Other 5 13%
Student > Ph. D. Student 5 13%
Researcher 4 10%
Student > Bachelor 3 8%
Student > Master 2 5%
Other 5 13%
Unknown 15 38%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 15%
Agricultural and Biological Sciences 4 10%
Chemistry 3 8%
Social Sciences 2 5%
Immunology and Microbiology 2 5%
Other 5 13%
Unknown 17 44%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 June 2017.
All research outputs
#14,940,583
of 22,979,862 outputs
Outputs from AMB Express
#348
of 1,239 outputs
Outputs of similar age
#188,664
of 317,259 outputs
Outputs of similar age from AMB Express
#24
of 70 outputs
Altmetric has tracked 22,979,862 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,239 research outputs from this source. They receive a mean Attention Score of 2.8. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 317,259 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 70 others from the same source and published within six weeks on either side of this one. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.