Title |
Carbamoylation correlates of cyanate neuropathy and cyanide poisoning: relevance to the biomarkers of cassava cyanogenesis and motor system toxicity
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Published in |
SpringerPlus, December 2013
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DOI | 10.1186/2193-1801-2-647 |
Pubmed ID | |
Authors |
Samuel Kimani, Victor Moterroso, Mike Lasarev, Sinei Kipruto, Fred Bukachi, Charles Maitai, Larry David, Desire Tshala-Katumbay |
Abstract |
We sought to elucidate the protein carbamoylation patterns associated with cyanate neuropathy relative to cyanide poisoning. We hypothesized that under a diet deficient in sulfur amino acids (SAA), the carbamoylation pattern associated with cyanide poisoning is similar to that of cyanate neuropathy. Male rats (6-8 weeks old) were fed a diet with all amino acids (AAA) or 75%-deficiency in SAA and treated with 2.5 mg/kg/body weight (bw) NaCN, or 50 mg/kg/bw NaOCN, or 1 μl/g/bw saline, for up to 6 weeks. Albumin and spinal cord proteins were analyzed using liquid chromatography mass spectrometry (LC-MS/MS). Only NaOCN induced motor deficits with significant levels of carbamoylation. At Day 14, we found a diet-treatment interaction effect on albumin carbamoylation (p = 0.07). At Day 28, no effect was attributed to diet (p = 0.71). Mean number of NaCN-carbamoylated sites on albumin was 47.4% higher relative to vehicle (95% CI:16.7-86.4%). Only NaOCN carbamoylated spinal cord proteins, prominently, under SAA-restricted diet. Proteins targets included myelin basic and proteolipid proteins, neurofilament light and glial fibrillary acidic proteins, and 2', 3' cyclic-nucleotide 3'-phosphodiesterase. Under SAA deficiency, chronic but not acute cyanide toxicity may share biomarkers and pathogenetic similarities with cyanate neuropathy. Prevention of carbamoylation may protect against the neuropathic effects of cyanate. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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India | 1 | 3% |
Unknown | 31 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 19% |
Student > Master | 4 | 13% |
Professor > Associate Professor | 4 | 13% |
Student > Doctoral Student | 3 | 9% |
Lecturer | 2 | 6% |
Other | 6 | 19% |
Unknown | 7 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 6 | 19% |
Agricultural and Biological Sciences | 4 | 13% |
Environmental Science | 3 | 9% |
Neuroscience | 3 | 9% |
Biochemistry, Genetics and Molecular Biology | 2 | 6% |
Other | 6 | 19% |
Unknown | 8 | 25% |